Portal de Periódicos da CAPES

Artigo Produção Nacional Revisado por pares

BIBTEX RIS

Low-intensity pulsed ultrasound (LIPUS) stimulates mineralization of MC3T3-E1 cells through calcium and phosphate uptake

2017; Elsevier BV; Volume: 84; Linguagem: Inglês

10.1016/j.ultras.2017.11.011

1874-9968

João Alberto Fioravante Tassinary, Adroaldo Lunardelli, Bruno de Souza Basso, Henrique Bregolin Dias, Anderson Velasque Catarina, Simone Stülp, Gabriela Viegas Haute, Bianca Andrade Martha, Denizar Alberto da Silva Melo, Fernanda Bordignon Nunes, Márcio Vinı́cius Fagundes Donadio, Jarbas Rodrigues de Oliveira,

Bone Tissue Engineering Materials

The present study aimed to evaluate the effect of low-intensity pulsed ultrasound (LIPUS) on pre-osteoblast mineralization using in vitro bioassays. Pre-osteoblastic MC3T3-E1 cells were exposed to LIPUS at 1 MHz frequency, 0.2 W/cm2 intensity and 20% duty cycle for 30 min. The analyses were carried out up to 336 h (14 days) after exposure. The concentration of collagen, phosphate, alkaline phosphatase, calcium and transforming growth factor beta 1 (TGF-β1) in cell supernatant and the presence of calcium deposits in the cells were analyzed. Our results showed that LIPUS promotes mineralized nodules formation. Collagen, phosphate, and calcium levels were decreased in cell supernatant at 192 h after LIPUS exposure. However, alkaline phosphatase and TGF-β1 concentrations remained unchanged. Therapeutic pulsed ultrasound is capable of stimulating differentiation and mineralization of pre-osteoblastic MC3T3-E1 cells by calcium and phosphate uptake with consequent hydroxyapatite formation.