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BIBTEX RIS

A standardized toolkit for genetic engineering of CTG clade yeasts

2017; Elsevier BV; Volume: 144; Linguagem: Inglês

10.1016/j.mimet.2017.11.015

1872-8359

Tatiana A. Defosse, Vincent Courdavault, Alix T. Coste, Marc Clastre, Thomas Dugé de Bernonville, Charlotte Godon, Patrick Vandeputte, Arnaud Lanoue, Antoine Touzé, Tomas Linder, Samir Droby, Carlos Augusto Rosa, Dominique Sanglard, Christophe d’Enfert, Jean‐Philippe Bouchara, Nathalie Giglioli‐Guivarc’h, Nicolas Papon,

Biotin and Related Studies

We have developed a series of synthetic constructs suitable to genetically manipulate a broad range of yeast species belonging to the fungal CTG clade. This molecular toolbox notably allows heterologous gene expression, single or dual fluorescence labeling and construction of luciferase-expressing strains for bioluminescence imaging.