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Genome sequence of the progenitor of the wheat D genome Aegilops tauschii

2017; Nature Portfolio; Volume: 551; Issue: 7681 Linguagem: Inglês

10.1038/nature24486

1476-4687

Ming‐Cheng Luo, Yong Q. Gu, Daniela Puiu, Hao Wang, Sven Twardziok, Karin R. Deal, Naxin Huo, Tingting Zhu, Le Wang, Yi Wang, Patrick E. McGuire, Shuyang Liu, Hai Long, Ramesh K. Ramasamy, J. Rodriguez, Sonny Lee Van, Luxia Yuan, Zhenzhong Wang, Zhiqiang Xia, Lichan Xiao, Olin D. Anderson, Shuhong Ouyang, Yong Liang, Aleksey V. Zimin, Geo Pertea, Peng Qi, Jeffrey L. Bennetzen, Xiongtao Dai, Matthew Dawson, Hansgeorg Müller, Karl Kugler, Lorena Rivarola‐Duarte, M. Spannagl, Klaus Mayer, Fu-Hao Lu, Michael W. Bevan, Philippe Leroy, Pingchuan Li, Frank M. You, Qixin Sun, Zhiyong Liu, Eric Lyons, Thomas Wicker, Steven L. Salzberg, Katrien M. Devos, Jan Dvořák,

Plant Disease Resistance and Genetics

A combination of advanced sequencing and mapping techniques is used to produce a reference genome of Aegilops tauschii, progenitor of the wheat D genome, providing a valuable resource for comparative genetic studies. Sequencing the genomes of crops plants provides useful resources for crop improvement and breeding. Jan Dvořák, Katrien Devos, Steven Salzberg and colleagues report a reference genome for Aegilops tauschii, the diploid progenitor of the D genome of hexaploid wheat. They use a combination of ordered-clone genome sequencing, whole-genome shotgun sequencing and BioNano optical genome mapping to assemble this large and highly repetitive genome. This provides a useful resource for comparative genomics studies of wheat. Aegilops tauschii is the diploid progenitor of the D genome of hexaploid wheat1 (Triticum aestivum, genomes AABBDD) and an important genetic resource for wheat2,3,4. The large size and highly repetitive nature of the Ae. tauschii genome has until now precluded the development of a reference-quality genome sequence5. Here we use an array of advanced technologies, including ordered-clone genome sequencing, whole-genome shotgun sequencing, and BioNano optical genome mapping, to generate a reference-quality genome sequence for Ae. tauschii ssp. strangulata accession AL8/78, which is closely related to the wheat D genome. We show that compared to other sequenced plant genomes, including a much larger conifer genome, the Ae. tauschii genome contains unprecedented amounts of very similar repeated sequences. Our genome comparisons reveal that the Ae. tauschii genome has a greater number of dispersed duplicated genes than other sequenced genomes and its chromosomes have been structurally evolving an order of magnitude faster than those of other grass genomes. The decay of colinearity with other grass genomes correlates with recombination rates along chromosomes. We propose that the vast amounts of very similar repeated sequences cause frequent errors in recombination and lead to gene duplications and structural chromosome changes that drive fast genome evolution.