Produção Nacional
Revisado por pares
Oocyte oxidative DNA damage may be involved in minimal/mild endometriosis‐related infertility
2017; Wiley; Volume: 85; Issue: 2 Linguagem: Inglês
10.1002/mrd.22943
ISSN1098-2795
AutoresMichele Gomes Da Broi, ALCEU AFONSO JORDÃO-JR, Rui Alberto Ferriani, Paula Andrea Navarro,
Tópico(s)Gynecological conditions and treatments
ResumoEarly endometriosis is associated with infertility, and oxidative stress may play a role in the pathogenesis of disease‐related infertility. This prospective case‐control study aimed to compare the presence of oxidative stress markers in the follicular microenvironment and systemic circulation of infertile women with minimal/mild endometriosis (EI/II) versus individuals undergoing controlled ovarian stimulation for intracytoplasmic sperm injection (ICSI). Seventy‐one blood samples (27 from infertile women with EI/II and 44 controls with tubal and/or male infertility factor) and 51 follicular fluid samples (19 EI/II and 32 controls) were obtained on the day of oocyte retrieval. Total hydroperoxides (FOX 1 ), reduced glutathione, vitamin E, Superoxide dismutase, total antioxidant capacity, malondialdehyde, advanced oxidation protein products, and 8‐hydroxy‐2′‐deoxyguanosine (8OHdG) concentrations were measured in both fluids. Women with EI/II showed higher FOX 1 (8.48 ± 1.72 vs. 7.69 ± 1.71 μmol/g protein) and lower total antioxidant capacity (0.38 ± 0.18 vs. 0.46 ± 0.15 mEq Trolox/L) concentrations in serum, and higher 8OHdG concentrations (24.21 ± 8.56 vs. 17.22 ± 5.6 ng/ml) in follicular fluid compared with controls. These data implicate both systemic and follicular oxidative stress may in infertile women with EI/II undergoing controlled ovarian stimulation for ICSI. Furthermore, the elevated 8OHdG concentrations in follicular fluid of women with EI/II may be related to compromised oocyte quality.
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