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Chronic Periodontitis and RANKL/OPG Ratio in Peri-Implant Mucosae Inflammation

2017; Fundação Odontológica de Ribeirão Preto; Volume: 29; Issue: 1 Linguagem: Inglês

10.1590/0103-6440201801241

1806-4760

Lucas Carneiro Costa, Marcos Alexandre da Fonseca, Aristides da Rosa Pinheiro, Telma Regina da Silva Aguiar, Aldir Nascimento Machado, Valquíria Quinelato, Letícia Ladeira Bonato, Diego Pinheiro Aguiar, Thays Vieira, Fernando Luiz Duarte de Almeida, Julie Calixto Lobo, Miriam Jordão, Priscilla Gonçalves Lomardo, José Mauro Granjeiro, Priscila Ladeira Casado,

Oral and gingival health research

tHistory of chronic periodontitis (CP) is a risk factor for oseointegration failure. The osteoclastogenesis system (RANK, RANKL and OPG) is critical for bone homeostatic control. We investigated the levels of OPG and RANKL in peri-implant tissues from volunteers with and without a history of CP and their association with mucosae inflammation. This is a single-blind case-contro study. Diagnosis of a history of CP and peri-implant examination was performed on 46 volunteers, divided into control (without history of CP, n=26) and CP group (with history of CP, n=20). Gingival biopsies were harvested during implant exposure. Quantitative PCR evaluated OPG/RANKL mRNA expressions. OPG and RANKL proteins were analyzed by western blot and immunohistochemistry assay. The chi-square test analyzed the significance of nominal variables between groups while continuous variables were analyzed by T-test or Mann-Whitney test, after Shapiro-Wilk test evaluation. The 2-ΔΔCT Livak method calculation evaluated the gene expression. Values of p<0.05 were considered statistically significant. Volunteers with CP history had 23 times higher chance of developing mucosae inflammation. High mucosae levels of RANKL (p=0.04) and RANKL/OPG (p=0.001) mRNA expressions were observed in CP group. CP volunteers showed increased RANKL protein levels in opposition to decreased OPG expression. Even without active periodontitis, volunteers with a history of CP had elevated gingival levels of RANKL/OPG and higher correlation with peri-implant mucosae inflammation and implant loss.