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Descripción de un foco de rangeliosis canina en el litoral noroeste del Uruguay

2017; Universidad de la República; Volume: 53; Issue: 208 Linguagem: Espanhol

10.29155/vet.23.208.1

0376-4362

Rodolfo Rivero,

Study of Mite Species

espanolLa rangeliosis canina es una enfermedad causada por el protozoario Rangelia vitalii y transmitida por garrapatas. Afecta caninos domesticos y silvestres, provocando un cuadro de anemia hemolitica y trombocitopenia. En Uruguay ha sido reportada en los Departamentos de Artigas, Salto y Treinta y Tres. El presente trabajo describe un foco de rangeliosis canina en el litoral oeste del Uruguay, diagnosticado mediante estudios epidemiologicos, clinicos, hematologicos, anatomopatologicos y tecnicas moleculares (PCR/secuenciacion). El estudio se baso en 9 casos de perros raza Griffon Fauve de Bretagne provenientes de un predio rural proximo a Quebracho, Departamento de Paysandu. De los animales ocho murieron y uno respondio al tratamiento. Los principales signos clinicos fueron apatia, ictericia, fiebre, diarrea con sangre y sangrado por narinas y punta de orejas. Hematolo- gicamente se constato anemia, trombocitopenia y leucocitosis. Los hallazgos macroscopicos se caracterizaron por palidez, ictericia generalizada, esplenomegalia, linfoadenomegalia y hepatomegalia. Al examen histopatologico se destaco: nefritis intersticial con degeneracion y necrosis tubular, miocarditis, necrosis hepatica centrolobulillar. Congestion y edema pulmonar, linfoadenitis, y en bazo infiltrado inflamatorio difuso perivascular de tipo mononuclear. En diversos organos se visualizaron zoitos de R. vitalii intracitoplasmaticas en las celulas endoteliales. En algunos animales asi como en los caniles de los perros se constato presencia de Amblyomma aureolatum. De dos casos se extrajo ADN de sangre y mediante PCR se amplifico un fragmento del gen 18s ARN ribosomal de Piroplasmas. La secuencia obtenida fue comparada con secuencias registradas en el GenBank utilizando la herramienta BLAST. El resultado revelo una homologia entre 99-100% con R. vitalii. Englishliosis is a disease caused by the protozoan Rangelia vitalii and transmitted by ticks. It affects domestic and wild canines, causing hemolytic anemia and thrombocytopenia. In Uruguay it has been reported in the following counties: Artigas, Salto and Treinta y Tres. The present study describes a canine rangeliosis outbreak in the northwest littoral of Uruguay, diagnosed by epidemiological, clinical, hematological, anatomopathological and molecular techniques (PCR / sequencing). The study was based on 9 cases of dogs, Fawn Brittany Griffon breed, used for hunting; from a farm near Quebracho, Paysandu County. Eight animals died and one responded to the treatment. The main clinical signs were apathy, jaundice, fever, bloody diarrhea and bleeding from nostrils and tips of the ears. The hematological findings were anemia, thrombocytopenia and leukocytosis. Main macroscopic lesions were characterized by paleness, generalized jaundice, splenomegaly, lymphadenomegaly, and hepatomegaly. The principal histopathological lesions were interstitial nephritis with tubular degeneration and necrosis, myocarditis, centrolobulillar hepatic necrosis, edematous and congestive lung, lymphoadenitis and perivascular mononuclear inflammatory infiltration in spleen. In several organs were observed R. vitalii zoites inside the cytoplasm of endothelial cells. In some animals and as well as in dog kennels were present Amblyomma aureolatum. DNA was extracted from blood of two cases and a fragment of the 18s ribosomal RNA gene of Piroplasm was amplified by PCR. The sequence obtained was compared to the sequences registered in the GenBank using the BLAST tool. The result showed a homology between 99-100% with R. vitalii.