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Partial Characterization of Citrus Leprosis Virus

1996; Volume: 13; Issue: 13 Linguagem: Inglês

10.5070/c51gp3336j

0074-7203

O. Lovisolo, Addolorata Colariccio, C. M. Chagas, Valeria Rossetti, Elliot Watanabe Kitajima, Ricardo Harakava,

Mosquito-borne diseases and control

Citrus leprosis virus (CiLV) was mechanically transmitted to the following nine plant species which all developed necrotic local lesions: sweet orange, Chenopodium amaranticolor, C. album, C. capitatum, C. foliosum, C. murale, C. polyspermum, C. quinoa and Gomphrena globosa.Mechanical transmission was improved by growing test plants a t temperatures above 25°C. Two isolates, one from sweet orange and the other from Cleopatra mandarin, were indistinguishable and were readily transmitted to C. quinoa, but could not be transmitted back to citrus from this host.In crude C. quinoa sap in PDET buffer, CiLV had a thermal inactivation point of 55-6O0C, a dilution end point of and a longevity in vitro at 4°C of 6 days.Attempts to purify CiLV from field citrus samples were unsuccessful.However, PEG-concentrated preparations contained a 25 kDa protein which was absent in extracts of healthy tissue from the same tree.In thin sections of infected sweet orange and Cleopatra mandarin leaves and young bark, bacilliform virus-like particles were detected in enclaves of the endoplasmic reticulum.Rounded structures were occasionally found in the perinuclear space of sweet orange leaf cells.These results support the view that CiLV may be a naked rhabdovirus.