Produção Nacional
Revisado por pares
Larvicidal Activity against Aedes aegypti and Chemical Characterization of the Inflorescences of Tagetes patula
2017; Hindawi Publishing Corporation; Volume: 2017; Linguagem: Inglês
10.1155/2017/9602368
ISSN1741-4288
AutoresLetícia Maria Krzyzaniak, Tânia Mara Antonelli-Ushirobira, Gean Pier Panizzon, Ana Luiza Sereia, José Roberto Pinto de Souza, João Antônio Cyrino Zequi, Cláudio Roberto Novello, Gisely Cristiny Lopes, Daniela Cristina de Medeiros Araújo, Denise Brentan Silva, Eneri Vieira de Souza Leite-Mello, João Carlos Palazzo de Mello,
Tópico(s)Phytochemical compounds biological activities
ResumoThe crude acetone extract (CAE) of defatted inflorescences of Tagetes patula was partitioned into five semipurified fractions: n-hexane (HF), dichloromethane (DF), ethyl acetate (EAF), n-butanol (BF), and aqueous (AQF). BF was fractionated by reversed-phase polyamide column chromatography, obtaining 34 subfractions, which were subjected to HSCCC, where patuletin and patulitrin were isolated. CAE and the fractions BF, EAF, DF, and AQF were analyzed by LC-DAD-MS, and patuletin and patulitrin were determined as the major substances in EAF and BF, respectively. BF was also analyzed by HPLC and capillary electrophoresis (CE), and patulitrin was again determined to be the main substance in this fraction. CAE and the semipurified fractions (750, 500, 300, 100, and 50 mg/L) were assayed for larvicidal activity against Aedes aegypti, with mortality rate expressed as percentage. All fractions except AQF showed insecticidal activity after 24 h exposure of larvae to the highest concentration. However, EAF showed the highest activity with more than 50% reduction in larval population at 50 mg/L. The insecticidal activity observed with EAF might have been due to the higher concentration of patuletin present in this fraction.
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