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Eph-ephrin signaling modulated by polymerization and condensation of receptors

2017; National Academy of Sciences; Volume: 114; Issue: 50 Linguagem: Inglês

10.1073/pnas.1713564114

1091-6490

Samuel Ojosnegros, Francesco Cutrale, Daniel Rodríguez, Jason Otterstrom, Chi Li Chiu, Verónica Hortigüela, Carolina Tarantino, Anna Seriola, Stephen Mieruszynski, Elena Martínez, Melike Lakadamyali, Ángel Raya, Scott E. Fraser,

Hippo pathway signaling and YAP/TAZ

Significance Cell communication is a precisely orchestrated mechanism in which cell receptors translate extracellular cues into intracellular signals. The Eph receptors act as a model guidance system steering cells to defined positions by their ligand ephrin. However, we still lack a mechanistic understanding of how membrane receptors can read a wide range of ligand concentrations and gradients and integrate them into coherent cellular responses. Here we reveal the evolution of Eph aggregation upon ephrin stimulation with unprecedented resolution by extending current imaging methods. The results fit biophysical models of protein aggregation. In these models, two protein oligomerization modes, polymerization and condensation, correlate with the “on/off” switching of the receptor activation, providing a precise, proportional, and dynamic response to variable ephrin inputs.