Produção Nacional
Revisado por pares
Bisphenol A reduces testosterone production in TM3 Leydig cells independently of its effects on cell death and mitochondrial membrane potential
2017; Elsevier BV; Volume: 76; Linguagem: Inglês
10.1016/j.reprotox.2017.12.002
ISSN1873-1708
AutoresGéssica Dutra Gonçalves, Simone Cristine Semprebon, Bruna Isabela Biazi, Mário Sérgio Mantovani, Glaura Scantamburlo Alves Fernandes,
Tópico(s)Toxic Organic Pollutants Impact
ResumoLeydig cells are the major testosterone-producing cells of the male reproductive system, and damage to these cells can impair fertility of men. Bisphenol A (BPA) is one of the chemicals with the highest volume of production worldwide. The aim of this study was to evaluate the effects of BPA on the growth, viability, and testosterone production of TM3 murine Leydig cells after exposure to BPA for 24 or 48 h. BPA reduced testosterone production, cell viability and cell growth in a concentration-dependent manner. The highest tested concentration of BPA (100 μM) increased cellular death, as indicated by an increased sub-G1 phase population and a larger number of cells labeled with Hoechst 3342. This concentration of BPA also decreased the number of metabolically active mitochondria as revealed by rhodamine staining. Therefore, our data show that BPA is toxic to Leydig TM3 cells and impairs their steroidogenic function.
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