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Lack of Truncated IFITM3 Transcripts in Cells Homozygous for the rs12252-C Variant That is Associated With Severe Influenza Infection

2017; Oxford University Press; Volume: 217; Issue: 2 Linguagem: Inglês

10.1093/infdis/jix512

1537-6613

Shokouh Makvandi‐Nejad, Henry Laurenson‐Schafer, Lili Wang, Dannielle Wellington, Yan Zhao, Boquan Jin, Ling Qin, Kerry Kite, Hooman K. Moghadam, Chaojun Song, Kevin Clark, Philip Hublitz, Alain Townsend, Hao Wu, Andrew J. McMichael, Yonghong Zhang, Tao Dong,

Viral Infections and Vectors

Interferon-induced transmembrane 3 (IFITM3) is known to restrict the entry of a range of enveloped viruses. The single nucleotide polymorphism rs12252-C within IFITM3 has been shown to be associated with severe influenza A virus infection. It has been suggested that rs12252-C results in expression of a truncated IFITM3 protein lacking the first 21 amino acids. By performing high-throughput RNA sequencing on primary dendritic cells and peripheral blood mononuclear cells isolated from pandemic H1N1 influenza and human immunodeficiency virus-1 (HIV-1) infected patients we show that full-length IFITM3 mRNA is dominantly expressed (>99%) across all rs12252 genotypes. Full-length IFITM3 protein can be detected in all genotypes.