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miR-30a suppresses lung cancer progression by targeting SIRT1

2017; Impact Journals LLC; Volume: 9; Issue: 4 Linguagem: Inglês

10.18632/oncotarget.23529

1949-2553

Yaowu Guan, Zhongming Rao, Cheng Chen,

Circular RNAs in diseases

// Yaowu Guan 1 , Zhongming Rao 1 and Cheng Chen 2 1 Department of Thoracic Surgery, Zhumadian Central Hospital, Zhumadian, Henan 463000, China 2 Department of Radiotherapy, Nanjing Medical University Affiliated Cancer Hospital, Cancer Institute of Jiangsu Province, Nanjing, Jiangsu 210009, China Correspondence to: Yaowu Guan, email: gyw0396@sina.cn Cheng Chen, email: njmudoctor@163.com Keywords: miR-30a; SIRT1; proliferation; apoptosis; invasion Abbreviations: SIRT1: silent information regulator 1; miRNA: microRNA Received: September 15, 2017 Accepted: December 04, 2017 Published: December 21, 2017 ABSTRACT The class III histone deacetylase silent information regulator 1 (SIRT1) is frequently overexpressed in a variety of tumors, including lung cancer; however, its regulatory mechanisms are largely unknown. In this study, we found that an inconsistent trend between SIRT1 protein and mRNA levels in human lung cancer tissues, suggesting that a post-transcriptional mechanism may involved in SIRT1 regulation. Because microRNAs are important post-transcriptional regulators of gene expression, candidate miRNAs that could potentially bind SIRT1 were gained through bioinformatics analyses. We further experimentally validated SIRT1 as the direct target of miR-30a by evaluating SIRT1 expression in lung cancer cells after the overexpression or knockdown of miR-30a and by luciferase assay. Moreover, we showed that miR-30a inhibited proliferation, invasion and promoted apoptosis of lung cancer cells by inhibiting SIRT1 in vitro and in vivo . Taken together, this study identified a new regulatory axis in which miR-30a and SIRT1 regulate the proliferation, invasion and apoptosis of lung cancer cells and lung tumorigenesis.