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Molecular Cloning and Sequencing of Coat Protein Genes of Citrus Tristeza Virus Isolated From Meyer Lemon and Homely Tangor Trees in Florida

1996; Volume: 13; Issue: 13 Linguagem: Inglês

10.5070/c55rs4s85g

0074-7203

Bayram Çevik, S. S. Pappu, H. R. Pappu, D. Tight, David Benscher, Stephen H. Futch, P. Rucks, R. F. Lee, C. L. Niblett,

Plant and Fungal Interactions Research

Samples were taken from seven Meyer lemon and two Homely tangor trees naturally infected with citrus tristeza virus (CTV) in Florida.The capsid protein gene (CPG) of CTV was amplified by reverse transcription/polyrnerase chain reaction, cloned in the pUC118 plasmid vector and sequenced.Comparative analyses of nucleotide and deduced amino acid sequences of the CPGs of the nine isolates showed 91-99% similarity among themselves and with T36, a Florida decline-inducing strain of CTV.Computer-assisted phylogenetic analyses of the capsid protein (CP) amino acid sequences indicated that the Meyer lemon isolates clustered either with T36 or with known stem pitting strains of CTV.However, isolates from Homely tangor clustered with T30 and other known mild isolates of CTV.Some of the Meyer lemon isolates showed stem pitting in Duncan grapefruit in biological assays.Variability among nucleotide sequences of some clones from the same isolate suggests that some trees may be infected with more than one strain of CTV.