Identification of a HSP40 gene involved in planarian regeneration
2017; Springer Nature; Volume: 72; Issue: 11 Linguagem: Inglês
10.1515/biolog-2017-0157
ISSN1336-9563
AutoresKe-Xue Ma, He-Cai Zhang, Yumei Zhang, Gege Song, Meng Wu, Guangwen Chen,
Tópico(s)Marine Ecology and Invasive Species
ResumoHeat shock protein 40 (HSP40), as an essential co-chaperone of HSP70, has been poorly investigated in planarians. In this study, a full-length cDNA of a HSP40 gene from planarian Dugesia japonica (designated Djhsp40) was identified and its expression pattern during planarian regeneration was examined. Its cDNA is 1,378 bp, which includes an open reading frame of 1,236 bp encoding 411 amino acids with a molecular weight of 46.2 kDa. DjHSP40 contains three conserved DNAJ domains belonging to the Type I HSP40/DNAJ family. After amputation is performed, Djhsp40 is up-regulated and the transcripts are specifically expressed in the blastema, and its inhibition by siRNA strongly delays the blastema growth. Interestingly, the silencing of Djhsp40 does not affect the expression level of neoblast-specific proliferation marker (Dj-mcm2 and DjpiwiB), but reduces Dj-mt-mmpA expression significantly. Our work provides a crucial clue for the study of the function of HSP40 in planarian regeneration.
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