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First Report of Red Dragon Fruit ( Hylocereus polyrhizus ) Anthracnose Caused by Colletotrichum siamense in China

2017; American Phytopathological Society; Volume: 102; Issue: 6 Linguagem: Inglês

10.1094/pdis-08-17-1193-pdn

1943-7692

Hongfan Zhao, S. C. Chen, Y. F. Chen, Chenchen Zou, X. L. Wang, Z. H. Wang, A. R. Liu, Golam Jalal Ahammed,

Botanical Research and Applications

HomePlant DiseaseVol. 102, No. 6First Report of Red Dragon Fruit (Hylocereus polyrhizus) Anthracnose Caused by Colletotrichum siamense in China PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Red Dragon Fruit (Hylocereus polyrhizus) Anthracnose Caused by Colletotrichum siamense in ChinaH. J. Zhao, S. C. Chen, Y. F. Chen, C. C. Zou, X. L. Wang, Z. H. Wang, A. R. Liu, and G. J. AhammedH. J. Zhao, S. C. Chen†Corresponding authors: A. R. Liu; E-mail: E-mail Address: evallyn@163.com and S. C. Chen; E-mail: E-mail Address: chen_shuangchen@126.com, Y. F. Chen, C. C. Zou, X. L. Wang, Z. H. Wang, A. R. Liu†Corresponding authors: A. R. Liu; E-mail: E-mail Address: evallyn@163.com and S. C. Chen; E-mail: E-mail Address: chen_shuangchen@126.com, and G. J. AhammedAffiliationsAuthors and Affiliations H. J. Zhao , College of Forestry, Henan University of Science and Technology, Luoyang, 471003, P.R. China S. C. Chen † , College of Forestry, Henan University of Science and Technology, Luoyang, 471003, P.R. China, and Department of Plant Science, Agricultural and Animal Husbandry College of Tibet University, Linzhi 860000, P.R. China Y. F. Chen C. C. Zou X. L. Wang , College of Forestry, Henan University of Science and Technology, Luoyang, 471003, P.R. China Z. H. Wang , Department of Plant Science, Agricultural and Animal Husbandry College of Tibet University, Linzhi 860000, P.R. China A. R. Liu † G. J. Ahammed , College of Forestry, Henan University of Science and Technology, Luoyang, 471003, P.R. China. Published Online:27 Mar 2018https://doi.org/10.1094/PDIS-08-17-1193-PDNAboutSectionsSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Dragon fruit (Hylocereus spp.) belonging to the family Cactaceae is an economically important fruit crop in China. Its importance is attributed to attractive color, pleasant taste, high nutrient content, and anticancer and antiaging properties in humans. In the spring and summer of 2016 and 2017, a stem disease was observed on H. polyrhizus cultivar Lianhua in a 15 ha commercial farm in Danzhou City of Hainan Province, China, affecting approximately 25% of the plants. Stems of H. polyrhizus showed spots or small, circular, faint pink-to-brown necrotic lesions that generally coalesced as symptoms progressed. About 35% of severely diseased young dragon fruit trees wilted and died in the orchard approximately 40 days after the appearance of initial anthracnose symptoms. Stems of symptomatic plants were surface disinfected in 0.1% sodium hypochlorite solution for 35 s, washed in distilled water, and dried on sterile filter paper. Then small stem fragments were cut from the margin of the affected tissues, plated on potato dextrose agar (PDA), and kept at 25°C and 12-h photoperiod. After 3 days of incubation, a single conidium was isolated and plated in PDA cultures for morphological characterization. Mycelial colonies showed abundant aerial mycelia, gray to brownish, with pink-colored masses of conidia. Conidia were hyaline, one-celled, guttulate, cylindrical, spindle-shaped with obtuse ends, measuring from 10.3 to 16.5 × 3.1 to 6.8 μm with both ends rounded. Cultural and conidial characteristics of the isolates were consistent with the description of Colletotrichum siamense (Prihastuti et al. 2009). The identity of a representative isolate, named CS22, was confirmed by means of a multilocus approach. Genomic DNA was extracted using the cetyl trimethylammonium bromide method. Regions from the internal transcribed spacer (ITS) and translation elongation factor-1 alpha (EF-1α) loci were amplified, sequenced with primers ITS-4 and ITS-5 (O'Donnell et al. 1998), EF-1 and EF-2 (O'Donnell et al. 1998), and AM-F and AM-R (Silva et al. 2012) and deposited in GenBank under accession numbers MF571889, MF571890, and MG657367, respectively. MegaBLAST analysis revealed that the ITS and TEF sequences were 99, 96, and 97% identical to C. siamense accessions numbers KR445673.1, KJ954494.1, and KC790590.1, respectively. The isolates were tested for pathogenicity by injecting conidial suspensions (1 × 106 conidia/ml) and pricking colonized toothpicks on 20 healthy H. polyrhizus 'Lianhua' stems. An equal number of stems serving as controls were mock inoculated with sterile distilled water. Inoculation points were sealed with sterile moist cotton. All inoculated plants and controls were placed in a greenhouse under temperatures of 30 to 35°C (day) and 23 to 28°C (night). Development of external symptoms on inoculated plants was observed every alternate day for 2 weeks. The stem lesion symptoms were visible on the infected plants and resembled those observed in the field, whereas control plants remained asymptomatic. C. siamense was reisolated only from the infected stems and identified by morphological identification and resequencing, thus fulfilling Koch's postulates. To our knowledge, this is the first report of C. siamense causing anthracnose on H. polyrhizus in China. The identification of this pathogen will allow the development of strategies for managing the disease in China.References:O'Donnell, K., et al. 1998. Proc. Natl. Acad. Sci. 95:2044. https://doi.org/10.1073/pnas.95.5.2044 Crossref, ISI, Google ScholarPrihastuti, H., et al. 2009. Fungal Divers. 39:89. ISI, Google ScholarSilva, D. N., et al. 2012. Mycologia 104:396. https://doi.org/10.3852/11-145 Crossref, ISI, Google ScholarDetailsFiguresLiterature CitedRelated Vol. 102, No. 6 June 2018SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 25 May 2018Published: 27 Mar 2018First Look: 27 Dec 2017Accepted: 24 Dec 2017 Pages: 1175-1175 Information© 2018 The American Phytopathological SocietyFundingFlexible Talent Introduction Project of Tibet Agriculture and Animal Husbandry CollegeGrant/Award Number: RXR201506Scientific Research Foundation for Postgraduate StudentGrant/Award Number: CXJJ-2016-ZR10National Natural Science Foundation of ChinaGrant/Award Number: 31471867International Scientific and Technological Cooperation Program in Henan ProvinceGrant/Award Number: 172102410050Cited byTwo Newly Identified Colletotrichum Species Associated with Mango Anthracnose in Central Thailand2 March 2023 | Plants, Vol. 12, No. 5Morphomolecular and cultural characteristics and host range of Lasiodiplodia theobromae causing stem canker disease in dragon fruit31 January 2022 | Journal of Basic Microbiology, Vol. 62, No. 6Fungal and Oomycete Diseases of Minor Tropical Fruit Crops11 April 2022 | Horticulturae, Vol. 8, No. 4First report of Colletotrichum tropicale on dragon fruit and the response of three Selenicereus species to anthracnose17 January 2022 | International Journal of Pest Management, Vol. 103Diaporthe species causing stem gray blight of red-fleshed dragon fruit (Hylocereus polyrhizus) in Malaysia16 February 2021 | Scientific Reports, Vol. 11, No. 1Colletotrichum species and complexes: geographic distribution, host range and conservation status29 September 2021 | Fungal Diversity, Vol. 110, No. 1Diversity of Colletotrichum Species Associated with Anthracnose Disease in Tropical Fruit Crops—A Review30 March 2021 | Agriculture, Vol. 11, No. 4Eight Colletotrichum Species, Including a Novel Species, Are Associated With Areca Palm Anthracnose in Hainan, ChinaXueren Cao, Xiangming Xu, Haiyan Che, Jonathan S. 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