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BIBTEX RIS

Genomic identification of microbial species adhering to maxillofacial prostheses and susceptibility to different hygiene protocols

2017; Taylor & Francis; Volume: 34; Issue: 1 Linguagem: Inglês

10.1080/08927014.2017.1403591

1029-2454

Juliana Barchelli Pinheiro, Marina Peris Vomero, Cássio do Nascimento, Evandro Watanabe, Helena de Freitas Oliveira Paranhos, Neide Pena Coto, Reinaldo Brito e Dias, Viviane de Cássia Oliveira, Cláudia Helena Silva‐Lovato,

Ear Surgery and Otitis Media

This study investigated the microbial colonization of maxillofacial prostheses and support tissues using the Checkerboard DNA–DNA hybridization method, and the efficacy of 0.12% chlorhexidine gluconate, 10% Ricinus communis solutions, or brushing, on colony forming unit (CFU) reduction in monospecies biofilms (Candida glabrata, Staphylococcus aureus, Streptococcus mutans, Escherichia coli, Enterococcus faecalis, and Pseudomonas aeruginosa) formed on two silicones (MDX 4-4210 and Bio-Skin). Biofilm was harvested from 43 maxillofacial prosthesis wearers for detection of 38 species of microorganisms. The CFU counts of the six above mentioned species were recorded after using the hygiene protocols. All 38 investigated species were identified in prostheses and tissues, with a higher prevalence in the prostheses. 0.12% chlorhexidine gluconate immersion showed the greatest antimicrobial effectiveness, followed by mechanical brushing protocols. MDX 4-4210 silicone produced lower CFU counts than Bio-Skin.