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Artigo Acesso aberto Produção Nacional Revisado por pares
BIBTEX RIS

Organic matter enrichment affects archaea community in limestone cave sediments

2017; BioMed Central; Volume: 79; Issue: 2 Linguagem: Inglês

10.4311/2016mb0138

ISSN

2331-3714

Autores

Eric de Lima Silva Marques, João Carlos Teixeira Dias, G.S. Silva, Carlos Priminho Pirovani, Rachel Passos Rezende,

Tópico(s)

Methane Hydrates and Related Phenomena

Resumo

Caves are unique environments filled with complex microbial communities that have adapted to oligotrophy.Communities of fungi and bacteria are commonly studied in touristic caves or are associated with guano or other sources of organic matter, but the archaeal community is often overlooked in these conditions.Based on this gap in the existing literature, the present study aims to evaluate the effect of a unique in vitro contamination event by organic matter in the archaeal community over the course of one year.For that purpose, samples were collected in Gruta Manoel Ioiô, a limestone cave located in Iraquara, Brazil.The collected samples were transported to the laboratory to undergo an enrichment of 0.25% or 0.5% mixture 1:1 (w/w) of yeast and meat extract.Samplings were collected at 0, 1, 6, and 12 months to evaluate the effects on the archaeal community by polymerase chain reaction followed by Denaturing Gel Gradient Electrophoresis (PCR-DGGE).PCR-DGGE profiles show that Operational Taxonomic Units (OTUs) remained in all samples, but variations were observed among the contaminated and control samples, especially at 6 months.Also, an increase in the number of OTUs was observed in samples that received the addition of organic matter in relation to the control.These OTUs were identified as Euryarchaeota and Crenarchaeota.This study showed that the archaeal community could be impacted by organic matter contamination in caves. introductionCaves are unique natural environments, usually characterized by the absence of light and by oligotrophy (Barton and Jurado, 2007;Simon et al., 2007).Despite those characteristics, caves present a high potential for microbial life that is little known, especially archaea (Gonzalez et al.,

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