Toward dynamic structural biology: Two decades of single-molecule Förster resonance energy transfer
2018; American Association for the Advancement of Science; Volume: 359; Issue: 6373 Linguagem: Inglês
10.1126/science.aan1133
ISSN1095-9203
AutoresEitan Lerner, Thorben Cordes, Antonino Ingargiola, Yazan Alhadid, Sangyoon Chung, Xavier Michalet, Shimon Weiss,
Tópico(s)Advanced Fluorescence Microscopy Techniques
ResumoClassical structural biology can only provide static snapshots of biomacromolecules. Single-molecule Förster resonance energy transfer (smFRET) paved the way for studying dynamics in macromolecular structures under biologically relevant conditions. Since its first implementation in 1996, smFRET experiments have confirmed previously hypothesized mechanisms and provided new insights into many fundamental biological processes, such as DNA maintenance and repair, transcription, translation, and membrane transport. We review 22 years of contributions of smFRET to our understanding of basic mechanisms in biochemistry, molecular biology, and structural biology. Additionally, building on current state-of-the-art implementations of smFRET, we highlight possible future directions for smFRET in applications such as biosensing, high-throughput screening, and molecular diagnostics.
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