Positive selection on the mitochondrial ATP synthase 6 and the NADH dehydrogenase 2 genes across 22 hare species (genus Lepus )
2018; Wiley; Volume: 56; Issue: 3 Linguagem: Inglês
10.1111/jzs.12204
ISSN1439-0469
AutoresHichem Ben Slimen, Asma Awadi, Zelalem Tolesa, Felix Knauer, Paulo C. Alves, Mohamed Makni, Franz Suchentrunk,
Tópico(s)Genetic diversity and population structure
ResumoJournal of Zoological Systematics and Evolutionary ResearchVolume 56, Issue 3 p. 428-443 ORIGINAL ARTICLE Positive selection on the mitochondrial ATP synthase 6 and the NADH dehydrogenase 2 genes across 22 hare species (genus Lepus) Hichem Ben Slimen, Corresponding Author Hichem Ben Slimen ben_slimen_hichem@yahoo.fr orcid.org/0000-0002-7351-9028 Unité de recherche Génomique des Insectes ravageurs des Cultures d'intérêt agronomique, Faculty of Sciences of Tunis, University of Tunis El Manar, Tunis, Tunisia Institut Supérieur de Biotechnologie de Béja, University of Jendouba, Béja, Tunisia Correspondence Hichem Ben Slimen Email: ben_slimen_hichem@yahoo.frSearch for more papers by this authorAsma Awadi, Asma Awadi Unité de recherche Génomique des Insectes ravageurs des Cultures d'intérêt agronomique, Faculty of Sciences of Tunis, University of Tunis El Manar, Tunis, TunisiaSearch for more papers by this authorZelalem Gebremariam Tolesa, Zelalem Gebremariam Tolesa Department of Biology, Hawassa University, Hawassa, EthiopiaSearch for more papers by this authorFelix Knauer, Felix Knauer Research Institute of Wildlife Ecology, University of Veterinary Medicine Vienna, Vienna, AustriaSearch for more papers by this authorPaulo Célio Alves, Paulo Célio Alves CIBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto, Vairão, PortugalSearch for more papers by this authorMohamed Makni, Mohamed Makni Unité de recherche Génomique des Insectes ravageurs des Cultures d'intérêt agronomique, Faculty of Sciences of Tunis, University of Tunis El Manar, Tunis, TunisiaSearch for more papers by this authorFranz Suchentrunk, Franz Suchentrunk Research Institute of Wildlife Ecology, University of Veterinary Medicine Vienna, Vienna, AustriaSearch for more papers by this author Hichem Ben Slimen, Corresponding Author Hichem Ben Slimen ben_slimen_hichem@yahoo.fr orcid.org/0000-0002-7351-9028 Unité de recherche Génomique des Insectes ravageurs des Cultures d'intérêt agronomique, Faculty of Sciences of Tunis, University of Tunis El Manar, Tunis, Tunisia Institut Supérieur de Biotechnologie de Béja, University of Jendouba, Béja, Tunisia Correspondence Hichem Ben Slimen Email: ben_slimen_hichem@yahoo.frSearch for more papers by this authorAsma Awadi, Asma Awadi Unité de recherche Génomique des Insectes ravageurs des Cultures d'intérêt agronomique, Faculty of Sciences of Tunis, University of Tunis El Manar, Tunis, TunisiaSearch for more papers by this authorZelalem Gebremariam Tolesa, Zelalem Gebremariam Tolesa Department of Biology, Hawassa University, Hawassa, EthiopiaSearch for more papers by this authorFelix Knauer, Felix Knauer Research Institute of Wildlife Ecology, University of Veterinary Medicine Vienna, Vienna, AustriaSearch for more papers by this authorPaulo Célio Alves, Paulo Célio Alves CIBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto, Vairão, PortugalSearch for more papers by this authorMohamed Makni, Mohamed Makni Unité de recherche Génomique des Insectes ravageurs des Cultures d'intérêt agronomique, Faculty of Sciences of Tunis, University of Tunis El Manar, Tunis, TunisiaSearch for more papers by this authorFranz Suchentrunk, Franz Suchentrunk Research Institute of Wildlife Ecology, University of Veterinary Medicine Vienna, Vienna, AustriaSearch for more papers by this author First published: 09 January 2018 https://doi.org/10.1111/jzs.12204Citations: 5 Contributing authors: Asma Awadi (awadiasma@gmail.com), Zelalem Gebremariam Tolesa (aslnp2000@yahoo.com), Felix Knauer (Felix.Knauer@vetmeduni.ac.at), Paulo Célio Alves (pcalves@fc.up.pt), Mohamed Makni (mdmakni98@gmail.com), Franz Suchentrunk (Franz.Suchentrunk@vetmeduni.ac.at) Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Abstract Amino acid changes in mitochondrial (mt) oxidative phosphorylation (OXPHOS) genes have been suggested as a key adaptation to environmental variation. Here, we analyzed 416 sequences of ATPase synthase 6 (MT-ATP6) and NADH dehydrogenase 2 (MT-ND2) in 22 different hare (Lepus) species from across a wide range of habitats and climates. We used site- and branch-based methods to test for positive selection on specific codons and lineages. We found four codons in MT-ATP6 and five in MT-ND2 under positive selection, affecting several species lineages. We investigated the association of protein variants at each locus with climate zone, using multinomial generalized linear models (glm), including species, regions, historical introgression events, and the co-occurring protein variant at the other locus as additional explanatory variables. A significant climate effect as based on the “Köppen climate classification” was observed for MT-ND2 protein variants as translated from our nucleotide sequences. Moreover, MT-ND2 protein variants were significantly affected by the co-occurring MT-ATP6 protein variant in the same mtDNA molecule. Contrary to the expectation for non-recombining mitochondrial DNA molecules, the presence of an evolutionarily relatively ancestral protein variant at one locus was associated with a relatively derived protein at the other locus in the same mitochondrial molecule, respectively. The relative evolutionary status of a protein variant was evaluated according to its positions relative to the respective out-group protein variant in a network analysis of nucleotide sequences. All our results suggest a complex effect of various climatic parameters acting on multiple mtOXPHOS genes in a co-adaptive way, favoring combinations of ancestral and derived variants. Citing Literature Supporting Information Filename Description jzs12204-sup-0001-SupInfo.pdfPDF document, 1,010.2 KB Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article. Volume56, Issue3August 2018Pages 428-443 RelatedInformation
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