Produção Nacional
Revisado por pares
Carbapenem-resistant Acinetobacter baumannii in Brazil: susceptibility profile and diversity of oxacillinases
2017; Sociedade Brasileira de Patologia Clínica; Volume: 53; Issue: 6 Linguagem: Inglês
10.5935/1676-2444.20170057
ISSN1678-4774
AutoresLisiane da Luz Rocha, Mariana Pagano, Juliana C. Campos, Jorge Luiz Mello Sampaio, Andreza Francisco Martins, Afonso Luís Barth,
Tópico(s)Vibrio bacteria research studies
ResumoIntroduction:The Acinetobacter calcoaceticus-baumannii (ABC) complex includes five species, and the A. baumannii is the most important of them because it carries mechanisms of carbapenems resistance, especially the oxacillinases.Objectives:The objectives of this study were to identify the species of the ABC complex, to evaluate the susceptibility profile and to investigate the presence of oxacillinases in carbapenems-resistant isolates from four Brazilian States.Methods: In the study period, 92 isolates from Rio Grande do Sul (RS), Rio de Janeiro (RJ), Paraná (PR) and São Paulo (SP) were collected.The isolates were identified by matrixassisted laser desorption ionization-time of fight mass spectrometry (MALDI-TOF MS) and sequencing of gyrB gene.Evaluation of susceptibility was performed by disk diffusion and broth microdilution.The presence of oxacillinases was performed by in-house multiplex polymerase chain reaction (PCR).Results: Ninety-one (99%) isolates were identified as A. baumannii by MALDI-TOF and sequencing.The majority of isolates (56; 61%) showed resistance to the six antimicrobial agents tested.Three isolates were resistant to polymyxin B [minimum inhibitory concentration (MIC) ≥ 4 μg/ml).Eighty (87%) isolates were positive to OXA-23-like, and twelve (13%) isolates to OXA-24-like.Conclusion: Our findings confirm the knowledge about the dissemination of the bla OXA-23 gene in Brazil and suggest the recent emergence and spread of bla OXA-24 gene, since it was identified in three of the four sampled states.
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