Produção Nacional
In Vivo Electrical Stimulation for the Assessment of Skeletal Muscle Contractile Function in Murine Models
2018; Springer Science+Business Media; Linguagem: Inglês
10.1007/978-1-4939-7614-0_26
ISSN1940-6029
AutoresKaio F. Vitzel, Marco Aurélio Salomão Fortes, Gabriel Nasri Marzuca-Nassr, Maria Vitória Martins Scervino, Carlos Hermano Pinheiro, Leonardo R. Silveira, Rui Curi,
Tópico(s)Neuroscience and Neural Engineering
ResumoSkeletal muscle electrical stimulation is commonly used for clinical purposes, assisting recovery, preservation, or even improvement of muscle mass and function in healthy and pathological conditions. Additionally, it is a useful research tool for evaluation of skeletal muscle contractile function. It may be applied in vitro, using cell culture or isolated fibers/muscles, and in vivo, using human subjects or animal models (neuromuscular electrical stimulation - NMES). This chapter focuses on the electrical stimulation of the sciatic nerve as a research method for evaluation of the contractile properties of murine hind limb muscles. Variations of this protocol allow for the assessment of muscle force, fatigue resistance, contraction and relaxation times, and can be used as a model of contraction-induced muscle injury, reactive oxygen species production, and muscle adaptation to contractile activity.
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