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Punica granatum L. Leaf Extract Attenuates Lung Inflammation in Mice with Acute Lung Injury

2018; Hindawi Publishing Corporation; Volume: 2018; Linguagem: Inglês

10.1155/2018/6879183

2314-8861

Aruanã Joaquim Matheus Costa Rodrigues Pinheiro, Jaciara Sá Gonçalves, Ádylla Wilenna Alves Dourado, Eduardo Martins de Sousa, Natilene Mesquita Brito, Lanna K. Silva, Marisa Cristina Aranha Batista, Joicy Cortez de Sá Sousa, Cinara Regina Aragão Vieira Monteiro, Elizabeth S. Fernandes, Valério Monteiro‐Neto, Lee Ann Campbell, Patrícia Maria Wiziack Zago, Lídio Gonçãlves Lima Neto,

Pomegranate: compositions and health benefits

The hydroalcoholic extract of Punica granatum (pomegranate) leaves was previously demonstrated to be anti-inflammatory in a rat model of lipopolysaccharide- (LPS-) induced acute peritonitis. Here, we investigated the anti-inflammatory effects of the ethyl acetate fraction obtained from the pomegranate leaf hydroalcoholic extract (EAFPg) on the LPS-induced acute lung injury (ALI) mouse model. Male Swiss mice received either EAFPg at different doses or dexamethasone (per os) prior to LPS intranasal instillation. Vehicle-treated mice were used as controls. Animals were culled at 4 h after LPS challenge, and the bronchoalveolar lavage fluid (BALF) and lung samples were collected for analysis. EAFPg and kaempferol effects on NO and cytokine production by LPS-stimulated RAW 264.7 macrophages were also investigated. Pretreatment with EAFPg (100–300 mg/kg) markedly reduced cell accumulation (specially neutrophils) and collagen deposition in the lungs of ALI mice. The same animals presented with reduced lung and BALF TNF- α and IL-1 β expression in comparison with vehicle controls ( p < 0.05 ). Additionally, incubation with either EAFPg or kaempferol (100 μ g/ml) reduced NO production and cytokine gene expression in cultured LPS-treated RAW 264.7 macrophages. Overall, these results demonstrate that the prophylactic treatment with EAFPg attenuates acute lung inflammation. We suggest this fraction may be useful in treating ALI.