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First Report of Lasiodiplodia theobromae Causing Branch Canker on ‘Manzanilla’ Olive in Northwestern Argentina

2017; American Phytopathological Society; Volume: 102; Issue: 3 Linguagem: Inglês

10.1094/pdis-07-17-0965-pdn

1943-7692

B. A. Pérez, J. Niz, Ricardo Salvador, Massimiliano Berretta, Mónica Esther María Roca, M. Pesce, A.C. Matías, M. González De Urreta, M. Paccioretti, Mariela Otero,

Yeasts and Rust Fungi Studies

HomePlant DiseaseVol. 102, No. 3First Report of Lasiodiplodia theobromae Causing Branch Canker on 'Manzanilla' Olive in Northwestern Argentina PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Lasiodiplodia theobromae Causing Branch Canker on 'Manzanilla' Olive in Northwestern ArgentinaB. A. Pérez, J. Niz, R. Salvador, M. Berretta, M. E. M. Roca, M. V. Pesce, A. C. Matías, M. González De Urreta, M. Paccioretti, and M. L. OteroB. A. Pérez†Corresponding author: B. A. Pérez; E-mail: E-mail Address: [email protected], J. Niz, R. Salvador, M. Berretta, M. E. M. Roca, M. V. Pesce, A. C. Matías, M. González De Urreta, M. Paccioretti, and M. L. OteroAffiliationsAuthors and Affiliations B. A. Pérez † J. Niz R. Salvador M. Berretta , INTA-CICVyA-IMYZA-Castelar, Argentina M. E. M. Roca , SENASA and Universidad Nacional de La Rioja, Argentina M. V. Pesce , IB-FI-Universidad Nacional de San Juan and CONICET, San Juan, Argentina A. C. Matías , INTA-EEA Catamarca, Argentina M. González De Urreta , INTA-Instituto de Biotecnología, Castelar, Argentina M. Paccioretti M. L. Otero , INTA-IPAVE, Córdoba, Argentina. Published Online:2 Jan 2018https://doi.org/10.1094/PDIS-07-17-0965-PDNAboutSections ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Olive is an economically important crop in Northwestern Argentina, where dead branches are frequently observed. In September of 2015 a total of 20 cankered branches and/or wood necrosis from 10 orchards showing dieback symptoms were collected from 'Manzanilla' olive trees in Catamarca (C) and La Rioja (LR) provinces. One cankered branch from each province was selected to study the dark brown internal tissue under the bark. Small pieces of symptomatic tissue were disinfected with 70% ethanol and 2% sodium hypochlorite, rinsed with sterile distilled water, aseptically transferred to 2% water agar (WA) plates, and incubated at 25°C under 12 h light-cycle period until fungal colonies developed. Pure culture of the fungus was obtained through successive transfers of hyphal tips from the margin of a growing colony onto WA plates, and then hyphal tips were transferred to 9-cm oatmeal agar (OA) plates to study morphological characteristics of the colony. Fungal colonies were fast growing and covered the plate surface after 4 days at 30°C in darkness. Colonies were dark blue to black, with abundant fluffy aerial mycelium and black underside. Grouped erumpent black pycnidia were observed after 15 days and measured 3 to 5 mm tall with conidia extruding in black masses. Conidiogenous cells were short and hyaline. Conidia were unicellular, hyaline, subovoid to ellipsoid, thick-walled with truncate base, and 25.94 (21 to 30) × 14.61 (12 to 15) µm (n = 40). Mature conidia were dark-walled, septate, with longitudinal striations, and 24.59 (22 to 32) × 13.88 (12 to 16) µm, and 22.54 (20 to 25) × 13.77 (13 to 15) µm (n = 40), with a length-to-width ratio of 1.77 and 1.64 for C and LR strains, respectively. Teleomorph was not observed in nature. Morphological traits agreed with descriptions of species within the Lasiodiplodia genus (Punithalingam 1976). To identify the isolates to species, the internal transcribed spacer ITS1-5.8S-ITS2 gene and part of the translation elongation factor (TEF1-α) and β-tubulin genes were amplified and sequenced using primers ITS1/ITS4, TEF1-α-688F/TEF1-α-986R, and Bet2a/Bet2b (Alves et al. 2008). ITS region (KY053847, KX816956), TEF gene (MF422024), and BET gene (MF628107) showed 100, 90, and 100% identity with L. theobromae isolates from 'Mission' and 'Sevillano' (JX515716, JX515717) and from Sevillano (JX515763, JX515685) olives from California, respectively (Úrbez-Torres et al. 2013). DNA sequences were deposited in GenBank. Two fungal isolates, one for each province, were used for pathogenicity tests. Isolates C and LR were grown on OA and incubated at 25°C under 12 h light for 7 days. Pathogenicity was verified on 10 healthy young 'Arbequina' olive plants. Three small wounds were made on the stem of each plant with a sterile needle. Mycelial plugs were placed on the wounded surface, covered with wet cotton, and kept under 100% relative humidity until symptom development. Noncolonized plugs were used as negative controls. Dark brown lesions were observed extending from the inoculation site reaching 3 cm after 30 days. Control plants showed no symptoms. Reisolation of the fungus from diseased parts showed the same morphological traits as the fungus isolated from the field. This fungus was previously described on olive trees in Italy (Carlucci et al. 2013) and California (Úrbez-Torres et al. 2013). This is the first report of L. theobromae on olive in Argentina.References:Alves, A., et al. 2008. Fungal Divers. 28:1. ISI, Google ScholarCarlucci, A., et al. 2013. Phytopathol. Mediterr. 52:517. ISI, Google ScholarPunithalingam, E. 1976. CMI Descriptions of Pathogenic Fungi and Bacteria, No. 519. CMI, Kew, Surrey, England. Google ScholarÚrbez-Torres, J. R., et al. 2013. Plant Dis. 97:231. https://doi.org/10.1094/PDIS-04-12-0390-RE Link, ISI, Google ScholarDetailsFiguresLiterature CitedRelated Vol. 102, No. 3 March 2018SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 19 Feb 2018Published: 2 Jan 2018First Look: 31 Oct 2017Accepted: 27 Oct 2017 Pages: 677-677 Information© 2018 The American Phytopathological SocietyCited byFirst Report of Lasiodiplodia theobromae Causing Leaf Rot on Agave angustifolia in MexicoGuadalupe Reyes-García, Santo Ángel Ortega-Acosta, Francisco Palemón-Alberto, Candelario Ortega-Acosta, Santiago Domínguez-Monge, José Terrones-Salgado, Álvaro Castañeda-Vildozola, Erubiel Toledo-Hernández, Blas Cruz-Lagunas, and Oscar Uriel Flores-Simon22 January 2023 | Plant Disease, Vol. 107, No. 2Sequencing and Functional Annotation of Competing Endogenous RNAs and MicroRNAs in Tea Leaves During Infection by Lasiodiplodia theobromaeDi Guo, Zhongqiu Xia, Xinyue Jiang, Hongke Huang, Yuqin Yang, Shilong Jiang, Delu Wang, and Zhuo Chen31 March 2022 | PhytoFrontiers™, Vol. 2, No. 3Functional Annotation of circRNAs in Tea Leaves After Infection by the Tea Leaf Spot Pathogen, Lasiodiplodia theobromaeYuanyou Yang, Xinyue Jiang, Jiayan Shi, Yu Wang, Honglin Huang, Yuqin Yang, Dongxue Li, Silong Jiang, Delu Wang, and Zhuo Chen3 February 2022 | Phytopathology®, Vol. 112, No. 2Lasiodiplodia theobromae (diplodia pod rot of cocoa)CABI Compendium, Vol. CABI CompendiumSequencing and Functional Annotation of mRNAs and lncRNAs from Tea (Camellia sinensis) Leaves During Infection by the Fungal Pathogen Lasiodiplodia theobromaeDongxue Li, Silong Jiang, Xiaodong Wen, Xingchen Song, Yuanyou Yang, Yu Wang, Honglin Huang, Delu Wang, and Zhuo Chen10 November 2021 | PhytoFrontiers™, Vol. 1, No. 4Integrated mRNA and Small RNA Sequencing for Analyzing Tea Leaf Spot Pathogen Lasiodiplodia theobromae, Under In Vitro Conditions and the Course of InfectionSilong Jiang, Qiaoxiu Yin, Dongxue Li, Xian Wu, Yong Wang, Delu Wang, and Zhuo Chen13 April 2021 | Phytopathology®, Vol. 111, No. 5Diversity and pathogenicity of Botryosphaeriaceae species associated with black root rot and stem cutting dry rot in Manihot esculenta in Brazil28 May 2020 | European Journal of Plant Pathology, Vol. 157, No. 3Bioprospecting for secondary metabolites of family Botryosphaeriaceae from a biotechnological perspective