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MicroRNA-21 and the Vulnerability of Atherosclerotic Plaques

2018; Elsevier BV; Volume: 26; Issue: 4 Linguagem: Inglês

10.1016/j.ymthe.2018.03.005

1525-0024

Temo Barwari, Marieke Rienks, Manuel Mayr,

Extracellular vesicles in disease

Stabilizing atherosclerotic lesions and preventing plaque ruptures can be seen as the Holy Grail in vascular medicine. In this issue of Molecular Therapy, Jin et al.1Jin H. Li D.Y. Chernogubova E. Sun C. Busch A. Eken S.M. Saliba-Gustafsson P. Winter H. Winski G. Raaz U. et al.Local Delivery of miR-21 Stabilizes Fibrous Caps in Vulnerable Atherosclerotic Lesions.Mol Ther. 2018; 26 (this issue): 1040-1055Abstract Full Text Full Text PDF PubMed Scopus (54) Google Scholar suggest the local delivery of microRNA-21 (miR-21) mimics as a potential therapeutic strategy to reduce plaque vulnerability. Interestingly, inhibition of this microRNA has also been proposed as a therapeutic approach for in-stent restenosis, where the application of anti-miR-21 oligonucleotides on stents improved vessel patency.2Wang D. Deuse T. Stubbendorff M. Chernogubova E. Erben R.G. Eken S.M. Jin H. Li Y. Busch A. Heeger C.-H. et al.Local microRNA modulation using a novel anti-miR-21-eluting stent effectively prevents experimental in-stent restenosis.Arterioscler. Thromb. Vasc. Biol. 2015; 35: 1945-1953Crossref PubMed Scopus (88) Google Scholar Atherosclerosis is a chronic inflammatory disease resulting from the build-up of lipid deposits in the innermost layer of an artery. Apart from lipids, atherosclerotic plaques comprise mainly smooth muscle cells (SMCs), macrophages, calcium, and fibrous connective tissue. Upon rupture or erosion of vulnerable atherosclerotic plaques, platelet aggregation results in thrombus formation that can occlude the vessel lumen. Stents are metallic scaffolds placed into the occluded segment of a diseased artery to hold it open. Current drug-eluting stents (DESs) are designed to inhibit vascular SMC proliferation to reduce neo-intima formation within the stent lumen. While greatly enhancing stent patency in coronary arteries, these anti-proliferative agents also inhibit the proliferation of endothelial cells, thus delaying re-endothelialization and arterial healing. After DES implantation, dual antiplatelet therapy is therefore required to prevent the inherent risk of thrombosis and stent occlusion. Alternatives to current DESs that would inhibit SMC proliferation without delaying re-endothelialization should result in less thrombogenicity. In the vasculature, miR-21 has previously been implicated as a determinant of SMC proliferation.3Ji R. Cheng Y. Yue J. Yang J. Liu X. Chen H. Dean D.B. Zhang C. MicroRNA expression signature and antisense-mediated depletion reveal an essential role of MicroRNA in vascular neointimal lesion formation.Circ. Res. 2007; 100: 1579-1588Crossref PubMed Scopus (812) Google Scholar, 4Wang M. Li W. Chang G.-Q. Ye C.-S. Ou J.-S. Li X.-X. Liu Y. Cheang T.-Y. Huang X.-L. Wang S.-M. MicroRNA-21 regulates vascular smooth muscle cell function via targeting tropomyosin 1 in arteriosclerosis obliterans of lower extremities.Arterioscler. Thromb. Vasc. Biol. 2011; 31: 2044-2053Crossref PubMed Scopus (127) Google Scholar, 5McDonald R.A. Halliday C.A. Miller A.M. Diver L.A. Dakin R.S. Montgomery J. McBride M.W. Kennedy S. McClure J.D. Robertson K.E. et al.Reducing in-stent restenosis: Therapeutic manipulation of miRNA in vascular remodeling and inflammation.J. Am. Coll. Cardiol. 2015; 65: 2314-2327Crossref PubMed Scopus (85) Google Scholar, 6McDonald R.A. White K.M. Wu J. Cooley B.C. Robertson K.E. Halliday C.A. McClure J.D. Francis S. Lu R. Kennedy S. et al.miRNA-21 is dysregulated in response to vein grafting in multiple models and genetic ablation in mice attenuates neointima formation.Eur. Heart J. 2013; 34: 1636-1643Crossref PubMed Scopus (54) Google Scholar For example, pharmacological and genetic miR-21 inhibition markedly decreased neo-intima formation in vein grafts5McDonald R.A. Halliday C.A. Miller A.M. Diver L.A. Dakin R.S. Montgomery J. McBride M.W. Kennedy S. McClure J.D. Robertson K.E. et al.Reducing in-stent restenosis: Therapeutic manipulation of miRNA in vascular remodeling and inflammation.J. Am. Coll. Cardiol. 2015; 65: 2314-2327Crossref PubMed Scopus (85) Google Scholar, 6McDonald R.A. White K.M. Wu J. Cooley B.C. Robertson K.E. Halliday C.A. McClure J.D. Francis S. Lu R. Kennedy S. et al.miRNA-21 is dysregulated in response to vein grafting in multiple models and genetic ablation in mice attenuates neointima formation.Eur. Heart J. 2013; 34: 1636-1643Crossref PubMed Scopus (54) Google Scholar and balloon-injured carotid arteries.3Ji R. Cheng Y. Yue J. Yang J. Liu X. Chen H. Dean D.B. Zhang C. MicroRNA expression signature and antisense-mediated depletion reveal an essential role of MicroRNA in vascular neointimal lesion formation.Circ. Res. 2007; 100: 1579-1588Crossref PubMed Scopus (812) Google Scholar These findings led to the exploration of local therapeutic strategies by coating stents with miR-21 inhibitors. When deployed in denuded arteries in rats, this approach prevented SMC-mediated in-stent restenosis without delaying re-endothelialization.2Wang D. Deuse T. Stubbendorff M. Chernogubova E. Erben R.G. Eken S.M. Jin H. Li Y. Busch A. Heeger C.-H. et al.Local microRNA modulation using a novel anti-miR-21-eluting stent effectively prevents experimental in-stent restenosis.Arterioscler. Thromb. Vasc. Biol. 2015; 35: 1945-1953Crossref PubMed Scopus (88) Google Scholar Conversely, SMC proliferation can also be beneficial in stabilizing the vessel wall and atherosclerotic plaques. For example, the proliferative effects of miR-21 mimics in SMCs reduced the expansion of abdominal aortic aneurysms.7Maegdefessel L. Azuma J. Toh R. Deng A. Merk D.R. Raiesdana A. Leeper N.J. Raaz U. Schoelmerich A.M. McConnell M.V. et al.MicroRNA-21 blocks abdominal aortic aneurysm development and nicotine-augmented expansion.Sci. Transl. Med. 2012; 4: 122ra22Crossref PubMed Scopus (229) Google Scholar The results presented by Jin et al.1Jin H. Li D.Y. Chernogubova E. Sun C. Busch A. Eken S.M. Saliba-Gustafsson P. Winter H. Winski G. Raaz U. et al.Local Delivery of miR-21 Stabilizes Fibrous Caps in Vulnerable Atherosclerotic Lesions.Mol Ther. 2018; 26 (this issue): 1040-1055Abstract Full Text Full Text PDF PubMed Scopus (54) Google Scholar in this issue propose the use of miR-21 mimics to enhance plaque stability. Unstable human plaques and atherosclerotic lesions in apolipoprotein E (apoE)-deficient mice (Apoe−/−) exhibit lower miR-21 levels. Based on in situ hybridization and immunohistochemistry, this expression pattern was ascribed to SMCs. The authors further show that plaques in Apoe/miR-21 double-deficient mice (Apoe−/−miR-21−/−) were more prone to rupture. Additionally, the unstable plaques were shown to harbor lower levels of the RE1-silencing transcription factor (REST). REST has been identified as a regulator of miR-21 while also being a direct target of miR-21. Subsequent in vitro analysis of SMCs revealed an anti-proliferative effect of REST, confirming the proliferative role of miR-21. Finally, local delivery of miR-21 mimics to carotid plaques using ultrasound-targeted microbubble destruction enhanced plaque stability. These findings suggest that, by increasing SMC proliferation, miR-21 mimics could stabilize the SMC-rich fibrous cap that shields the lipid-filled core of atherosclerotic plaques. As is the case for most miRNAs, miR-21 is ubiquitously expressed.8Barwari T. Joshi A. Mayr M. MicroRNAs in cardiovascular disease.J. Am. Coll. Cardiol. 2016; 68: 2577-2584Crossref PubMed Scopus (262) Google Scholar It is particularly abundant in circulating hematopoietic cells, where it appears to act as an "emergency brake" on inflammation. In macrophages, miR-21 directly targets the pro-inflammatory programmed cell death protein 4 (PDCD4), thereby increasing the secretion of anti-inflammatory interleukin-10 (IL-10).9Sheedy F.J. Palsson-McDermott E. Hennessy E.J. Martin C. O'Leary J.J. Ruan Q. Johnson D.S. Chen Y. O'Neill L.A. Negative regulation of TLR4 via targeting of the proinflammatory tumor suppressor PDCD4 by the microRNA miR-21.Nat. Immunol. 2010; 11: 141-147Crossref PubMed Scopus (787) Google Scholar miR-21 also directly targets phosphatase and tensin homolog (PTEN), steering macrophages toward a reparative phenotype that promotes resolution of inflammation and tissue recovery.10Das A. Ganesh K. Khanna S. Sen C.K. Roy S. Engulfment of apoptotic cells by macrophages: a role of microRNA-21 in the resolution of wound inflammation.J. Immunol. 2014; 192: 1120-1129Crossref PubMed Scopus (225) Google Scholar Thus, miR-21 may also modulate tissue inflammation, in part, through monocyte differentiation toward an anti-inflammatory macrophage phenotype. A recent study by Canfrán-Duque et al.11Canfrán-Duque A. Rotllan N. Zhang X. Fernández-Fuertes M. Ramírez-Hidalgo C. Araldi E. Daimiel L. Busto R. Fernández-Hernando C. Suárez Y. Macrophage deficiency of miR-21 promotes apoptosis, plaque necrosis, and vascular inflammation during atherogenesis.EMBO Mol. Med. 2017; 9: 1244-1262Crossref PubMed Scopus (136) Google Scholar also focused on miR-21 in the context of atherosclerosis. miR-21 accumulated in murine atherosclerotic plaques along with CD68, a macrophage marker. Deficiency of miR-21 in bone marrow cells promoted vascular inflammation and plaque necrosis in low-density lipoprotein receptor (LDLr) null mice. Compared to wild-type bone marrow, transplantation of miR-21−/− bone marrow into Ldlr−/− mice resulted in larger and less stable atherosclerotic plaques due to increased inflammatory cell infiltration. Thus, miR-21 expression in murine hematopoietic cells attenuates vascular inflammation. The findings of Jin et al.1Jin H. Li D.Y. Chernogubova E. Sun C. Busch A. Eken S.M. Saliba-Gustafsson P. Winter H. Winski G. Raaz U. et al.Local Delivery of miR-21 Stabilizes Fibrous Caps in Vulnerable Atherosclerotic Lesions.Mol Ther. 2018; 26 (this issue): 1040-1055Abstract Full Text Full Text PDF PubMed Scopus (54) Google Scholar also highlight the impact of miR-21 on the influx of macrophages. Apoe−/−miR-21−/− mice displayed more advanced plaque formation at an early age, with a concomitant increase of macrophage infiltration and foam cell formation. In line with the recent findings of Canfrán-Duque et al.,11Canfrán-Duque A. Rotllan N. Zhang X. Fernández-Fuertes M. Ramírez-Hidalgo C. Araldi E. Daimiel L. Busto R. Fernández-Hernando C. Suárez Y. Macrophage deficiency of miR-21 promotes apoptosis, plaque necrosis, and vascular inflammation during atherogenesis.EMBO Mol. Med. 2017; 9: 1244-1262Crossref PubMed Scopus (136) Google Scholar miR-21 null peritoneal macrophages displayed an increase in oxidized LDL uptake and foam cell formation through enhanced nuclear factor κB (NF-κB) signaling. Notably, secreted factors from Apoe−/−miR-21−/− macrophages could suppress SMC proliferation in vitro. A common challenge in miRNA research is to identify the cell types that are responsible for the observed therapeutic benefits. Although the present study confirms a mechanistic link between miR-21 and SMC proliferation, it remains unclear whether the beneficial effect relies predominantly on SMCs, macrophages, or other cell types (Figure 1). Using a multi-omics approach, we recently identified a protein signature of symptomatic atherosclerotic plaques that implied a shared involvement of immune cells and SMCs.12Langley S.R. Willeit K. Didangelos A. Matic L.P. Skroblin P. Barallobre-Barreiro J. Lengquist M. Rungger G. Kapustin A. Kedenko L. et al.Extracellular matrix proteomics identifies molecular signature of symptomatic carotid plaques.J. Clin. Invest. 2017; 127: 1546-1560Crossref PubMed Scopus (74) Google Scholar Also, the plasticity of SMCs is currently under debate, with lineage tracing experiments suggesting a potential SMC origin of macrophage-like cell types.13Shankman L.S. Gomez D. Cherepanova O.A. Salmon M. Alencar G.F. Haskins R.M. Swiatlowska P. Newman A.A.C. Greene E.S. Straub A.C. et al.KLF4-dependent phenotypic modulation of smooth muscle cells has a key role in atherosclerotic plaque pathogenesis.Nat. Med. 2015; 21: 628-637Crossref PubMed Scopus (656) Google Scholar, 14Bennett M.R. Sinha S. Owens G.K. Vascular smooth muscle cells in atherosclerosis.Circ. Res. 2016; 118: 692-702Crossref PubMed Scopus (1124) Google Scholar Finally, apoE mediates the reverse cholesterol transport,15Zanotti I. Pedrelli M. Potì F. Stomeo G. Gomaraschi M. Calabresi L. Bernini F. Macrophage, but not systemic, apolipoprotein E is necessary for macrophage reverse cholesterol transport in vivo.Arterioscler. Thromb. Vasc. Biol. 2011; 31: 74-80Crossref PubMed Scopus (58) Google Scholar and atherosclerosis is known to differ between man and apoE null mice.16Allahverdian S. Pannu P.S. Francis G.A. Contribution of monocyte-derived macrophages and smooth muscle cells to arterial foam cell formation.Cardiovasc. Res. 2012; 95: 165-172Crossref PubMed Scopus (114) Google Scholar Despite these notes of caution, the study by Jin et al.1Jin H. Li D.Y. Chernogubova E. Sun C. Busch A. Eken S.M. Saliba-Gustafsson P. Winter H. Winski G. Raaz U. et al.Local Delivery of miR-21 Stabilizes Fibrous Caps in Vulnerable Atherosclerotic Lesions.Mol Ther. 2018; 26 (this issue): 1040-1055Abstract Full Text Full Text PDF PubMed Scopus (54) Google Scholar advances our insight into the role of miR-21 in vascular biology. This is a timely contribution as miRNA-based therapies progress to clinical application. Clinical trials are currently underway in patients with Alport syndrome, where systemic miR-21 inhibition is evaluated for the treatment of renal fibrosis. A better understanding of the effects of miR-21 in the context of other diseases is therefore highly relevant. The findings of Jin et al.1Jin H. Li D.Y. Chernogubova E. Sun C. Busch A. Eken S.M. Saliba-Gustafsson P. Winter H. Winski G. Raaz U. et al.Local Delivery of miR-21 Stabilizes Fibrous Caps in Vulnerable Atherosclerotic Lesions.Mol Ther. 2018; 26 (this issue): 1040-1055Abstract Full Text Full Text PDF PubMed Scopus (54) Google Scholar implicate that local therapy with miR-21 may indeed achieve higher drug concentrations at the target site and could minimize the risk of systemic side effects from miRNA therapeutics. Nevertheless, even local therapy for ubiquitously expressed miRNAs could have potential systemic effects, for example, by affecting circulating cells in the blood stream. T.B. is funded by an Interdisciplinary Studentship of the King's British Heart Foundation Centre. M.M. is a British Heart Foundation (BHF) Chair Holder (CH/16/3/32406) with BHF programme grant support (RG/16/14/32397) and member of a network on "MicroRNA-based Therapeutic Strategies in Vascular Disease" funded by the Fondation Leducq. He is also supported by an excellent initiative (Competence Centers for Excellent Technologies [COMET]) of the FFG (Austrian Research Promotion Agency): Research Center of Excellence in Vascular Ageing-Tyrol, VASCage (K- Project No. 843536) funded by BMVIT (Federal Ministry for Transport, Innovation and Technology), BMWFW (federal Ministry of Science, Research and Economy), the Wirtschaftsagentur Wien, and Standortagentur Tirol. Local Delivery of miR-21 Stabilizes Fibrous Caps in Vulnerable Atherosclerotic LesionsJin et al.Molecular TherapyJanuary 17, 2018In BriefAtherosclerosis is a common feature of vascular diseases, with its advancement having severe consequences such as myocardial infarction or stroke. The current work by Jin et al. not only identifies miR-21 as a main regulator of atherosclerotic lesion instability but also indicates that its local overexpression can stabilize advanced plaques. Full-Text PDF Open Archive