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Graphene immunosensors for okadaic acid detection in seawater

2018; Elsevier BV; Volume: 138; Linguagem: Inglês

10.1016/j.microc.2018.01.041

1095-9149

Jéssica Antunes, Celine I.L. Justino, João Pinto da Costa, Susana Cardoso, Armando C. Duarte, Teresa Rocha‐Santos,

Advanced biosensing and bioanalysis techniques

In this work, we report the fabrication of immunosensors based on field effect transistors with graphene (Gr-FET) for the detection of okadaic acid (OA) in real seawater samples. OA is a marine toxin, and specifically a diarrheic shellfish poison produced by the dinoflagellate species Dinophysis and Prorocentrum. The analytical results obtained with fabricated Gr-FET were compared with those obtained with a traditional methodology (enzyme-linked immunosorbent assay, ELISA) in order to validate the immunosensors for OA detection. Standard solutions of OA with concentrations between 0.05 and 300 ng mL−1 were tested to construct the calibration curve and four spiked real seawater samples were used to validate the immunosensors. With the obtained good reproducibility (0.54–2.19%) and repeatability (0.05–2.06%) as well as low limit of detection (0.05 ng mL−1) and adequate recovery range (98.2–100.7%), the proposed immunosensors are useful for the detection of OA in real seawater samples and could be advantageously extended for detection of other toxins or agents of marine environmental pollution.