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Detection of amyloid fibrils in Parkinson’s disease using plasmonic chirality

2018; National Academy of Sciences; Volume: 115; Issue: 13 Linguagem: Inglês

10.1073/pnas.1721690115

1091-6490

Jatish Kumar, Hasier Eraña, Elena López‐Martínez, Nathalie Claes, Víctor F. Martín, Diego M. Solís, Sara Bals, Aitziber L. Cortajarena, Joaquı́n Castilla, Luis M. Liz‐Marzán,

Advanced Neuroimaging Techniques and Applications

Significance This contribution reports on the application of gold nanorods to the detection of amyloids in Parkinson’s and prion diseases. We found that gold nanorods show no interaction with monomeric proteins but adsorb onto helical protein fibrils. Chiral amyloid templates induce helical arrangement of nanorods, giving rise to intense optical activity at the plasmon resonance wavelengths. This report shows the use of protein fibrils as templates for chiral nanoparticle assembly and development of a biodetection technique. We show this effect on a model recombinant protein, α-synuclein (involved in Parkinson’s disease), using CD, cryogenic transmission EM tomography, and theoretical simulations supporting the experimental findings. We additionally show application to identify patients with Parkinson’s disease from human brain homogenates.