Produção Nacional
Revisado por pares
First Report of Sida micrantha mosaic virus Infecting Oxalis Species in Brazil
2018; American Phytopathological Society; Volume: 102; Issue: 9 Linguagem: Inglês
10.1094/pdis-01-18-0149-pdn
ISSN1943-7692
AutoresRafaela S. Fontenele, Gabriel de Oliveira Ribeiro, N. S. Lamas, Simone G. Ribeiro, Alexandre Florian da Costa, L. S. Boiteux, M. E. N. Fonseca,
Tópico(s)Plant Pathogenic Bacteria Studies
ResumoHomePlant DiseaseVol. 102, No. 9First Report of Sida micrantha mosaic virus Infecting Oxalis Species in Brazil PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Sida micrantha mosaic virus Infecting Oxalis Species in BrazilR. S. Fontenele, G. C. Ribeiro, N. S. Lamas, S. G. Ribeiro, A. F. Costa, L. S. Boiteux, and M. E. N. FonsecaR. S. Fontenele, G. C. Ribeiro, N. S. Lamas, S. G. Ribeiro†Corresponding author: S. G. Ribeiro; E-mail: E-mail Address: simone.ribeiro@embrapa.brhttp://orcid.org/0000-0002-4965-1424, A. F. Costa, L. S. Boiteuxhttp://orcid.org/0000-0002-5791-8002, and M. E. N. FonsecaAffiliationsAuthors and Affiliations R. S. Fontenele G. C. Ribeiro N. S. Lamas S. G. Ribeiro † , Embrapa Recursos Genéticos e Biotecnologia, Brasília - DF, Brazil A. F. Costa L. S. Boiteux M. E. N. Fonseca , Embrapa Hortaliças, Brasília - DF, Brazil. Published Online:29 Jun 2018https://doi.org/10.1094/PDIS-01-18-0149-PDNAboutSections ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Species of Oxalis (family Oxalidaceae) are widely distributed in subtropical areas of Brazil and found as weeds in agricultural areas and urban gardens. In Brazil, Oxalis oxyptera was reported as a geminivirus host in the 1970s (Costa and Betti 1978). However, currently, there is an overall lack of information about geminiviruses infecting Oxalis in the country. Oxalis spp. exhibiting golden spots and yellow mosaic symptoms were collected in surveys carried out from 2003 to 2015 in Brazil. Seventeen symptomatic and five symptomless leaf samples were collected from Oxalis plants occurring as weeds within tomato fields in three growing areas: Gama and Rajadinha (the Federal District, DF) and Formosa, Goiás State. The incidence of plants with symptoms in the fields was around 5%. Five symptomatic samples were also collected in urban gardens, three in Brasília - DF and two in Londrina, Paraná State (PR). The infection by a bipartite begomovirus was tested via polymerase chain reaction (PCR) using primers for DNA-A and DNA-B (Rojas et al. 1993) and confirmed only in the symptomatic Oxalis plants. Sequence analysis of the partial DNA-A amplicons (∼1,120 bp) of these viruses indicated nucleotide sequence identity ranging from 95 to 99% with Sida micrantha mosaic virus (SiMMV). The DNA from four isolates (three from DF and one from PR) were used as templates for rolling circle amplification (RCA) using Phi-29 DNA polymerase. RCA products were digested with the endonucleases EcoRI to obtain the DNA-A and ClaI, SacI, and HindIII to obtain the DNA-B. Digestion products of ∼2.6 kb were cloned into pBluescript SK+ and sequenced at Macrogen (South Korea). DNA-A clones (GenBank accession nos. KY650717 and KY650722) shared 93% nucleotide identity, and Species Demarcation Tool analysis resulted in identities ranging from 91 to 99% with the 28 SiMMV DNA-A sequences available at GenBank. DNA-B clones (GenBank accession nos. KY650716, KY650718 to KY650721, and KY650723) shared 88 to 100% identity and had identities ranging from 86 to 96% with all 34 SiMMV DNA-B sequences at GenBank. Transmission tests gave positive results by using Bemisia tabaci MEAM-1 adults fed in a field-collected SiMMV-infected O. oxyptera (acquisition access period of 48 h) and then transferred to healthy O. oxyptera transplants (inoculation access period of 48 h). Typical golden leaf spot symptoms appeared in seven out of 10 O. oxyptera test plants about 15 days after inoculation, and SiMMV infection was confirmed by PCR and Sanger sequencing. The ornamental species O. debilis was previously reported as a host of a new begomovirus (Oxalis yellow vein virus) in the United States (Herrera et al. 2015). However, to our knowledge, this is the first report of Oxalis species as natural hosts of SiMMV in South America. These stolon-bearing weeds might represent persistent inoculum sources of SiMMV to malvaceous crops (cotton and okra), as well as to tomatoes, soybeans, and snap beans, which are sporadically infected by this virus in Brazil (Fernandes et al. 2009; Fernandes-Acioli et al. 2011).References:Costa, A. S., and Betti, J. A. 1978. Summa Phytopathol. 4:3. Google ScholarFernandes, F. R., et al. 2009. Arch. Virol. 154:1567. https://doi.org/10.1007/s00705-009-0463-0 Crossref, ISI, Google ScholarFernandes-Acioli, N. A. N., et al. 2011. Plant Dis. 95:1196. https://doi.org/10.1094/PDIS-05-10-0343 Link, ISI, Google ScholarHerrera, F., et al. 2015. Eur. J. Plant Pathol. 142:203. https://doi.org/10.1007/s10658-015-0594-y Crossref, ISI, Google ScholarRojas, M. R., et al. 1993. Plant Dis. 77:340. https://doi.org/10.1094/PD-77-0340 Crossref, ISI, Google ScholarFunding: Funding was provided by Fundação de Apoio à Pesquisa do Distrito Federal, Conselho Nacional de Desenvolvimento Científico e Tecnológico, and Empresa Brasileira de Pesquisa Agropecuária.DetailsFiguresLiterature CitedRelated Vol. 102, No. 9 September 2018SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 20 Aug 2018Published: 29 Jun 2018First Look: 20 Mar 2018Accepted: 17 Mar 2018 Page: 1862 Information© 2018 The American Phytopathological SocietyFundingFundação de Apoio à Pesquisa do Distrito FederalConselho Nacional de Desenvolvimento Científico e TecnológicoEmpresa Brasileira de Pesquisa AgropecuáriaCited byNovel natural hosts of tomato severe rugose virus (ToSRV) in the Fabaceae, Solanaceae, and Oxalidaceae families19 October 2021 | Journal of Plant Diseases and Protection, Vol. 129, No. 2Oxalis (wood sorrels)CABI Compendium, Vol. CABI CompendiumSida micrantha mosaic virusCABI Compendium, Vol. CABI Compendium
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