Produção Nacional
Revisado por pares
Occurrence of Bipolaris oryzae Causing Leaf Spot on Brachypodium distachyon in Brazil
2018; American Phytopathological Society; Volume: 102; Issue: 7 Linguagem: Inglês
10.1094/pdis-11-17-1806-pdn
ISSN1943-7692
AutoresTiago Edu Kaspary, Cristiano Bellé, Renata Moccellin, Luan Cutti, Carlos Alberto Gonsiorkiewicz Rigon, Aldo Merotto, C. R. J. de Farias,
Tópico(s)Plant Pathogens and Resistance
ResumoHomePlant DiseaseVol. 102, No. 7Occurrence of Bipolaris oryzae Causing Leaf Spot on Brachypodium distachyon in Brazil PreviousNext DISEASE NOTES OPENOpen Access licenseOccurrence of Bipolaris oryzae Causing Leaf Spot on Brachypodium distachyon in BrazilT. E. Kaspary, C. Bellé, R. Moccellin, L. Cutti, C. A. G. Rigon, A. Merotto Junior, and C. R. J. de FariasT. E. KasparySearch for more papers by this author, C. Bellé†Corresponding author: C. Bellé; E-mail: E-mail Address: crbelle@gmail.comhttp://orcid.org/0000-0003-2247-3207Search for more papers by this author, R. MoccellinSearch for more papers by this author, L. CuttiSearch for more papers by this author, C. A. G. RigonSearch for more papers by this author, A. Merotto JuniorSearch for more papers by this author, and C. R. J. de FariasSearch for more papers by this authorAffiliationsAuthors and Affiliations T. E. Kaspary , Universidade Federal do Rio Grande do Sul, Departamento de Fitotecnia, 91540-000, Porto Alegre, RS, Brazil C. Bellé † R. Moccellin , Universidade Federal de Pelotas, Departamento de Fitossanidade, 96010-900, Capão do Leão, RS, Brazil L. Cutti C. A. G. Rigon A. Merotto Junior , Universidade Federal do Rio Grande do Sul, Departamento de Fitotecnia, Porto Alegre, RS, Brazil C. R. J. de Farias , Universidade Federal de Pelotas, Departamento de Fitossanidade, Capão do Leão, RS, Brazil. Published Online:26 Apr 2018https://doi.org/10.1094/PDIS-11-17-1806-PDNAboutSectionsSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Brachypodium distachyon L., family Poaceae, is native to southern Europe, where it inhabits forests, roadsides, and crop fields as well as other dry environments. B. distachyon is a model plant for genetic and molecular biology studies, and has recently been used in grass disease studies because of a close phylogenetic relationship with major cereal crops and a susceptibility to many important monocot pathogens (Fitzgerald et al. 2015). In September 2017, symptoms of leaf spots were observed on B. distachyon in Porto Alegre, Rio Grande do Sul state, Brazil. Diseased plants exhibited leaves with brown, often large, elliptical, necrotic spots up to 5 × 50 mm covering as much as 75% of affected plants. Lesions were surrounded by a diffuse chlorotic margin, and larger lesions had tan centers. Leaf lesion samples were surface sterilized (70% ethanol for 30 s, 1% NaClO for 2 min, rinsed three times in sterile water, dried on sterilized filter paper) and placed on potato dextrose agar (PDA). Colonies on PDA were initially gray, with unevenly distributed mycelial resulting in a fluffy mycelium, and dark gray with age. Conidiophores were brown, straight, cylindrical, and multiseptate. Conidia (n = 100) were brown, curved, ellipsoidal tapering to rounded ends, and measured 61.4 to 89.9 µm (length) × 20.4 to 31.6 µm (width) (mean 75.1 × 23.7 µm), with 3 to 10 septa. The specimen was deposited in the Federal University of Pelotas Herbarium (LPS01-29). To further confirm the identification, the ITS, GPD, and EF1-α genes were amplified with PCR using primer pairs ITS1/ITS4, GPD1/GPD2, and EF983/EF2218R, respectively (Manamgoda et al. 2014). Sequences of the obtained DNA regions were submitted to GenBank (accession nos. ITS: MG448606; GPD: MG458233; and EF1-α: MG458234). BLAST searches in GenBank indicated 99 to 100% identity with B. oryzae (Breda de Haan) Shoemaker sequences. To confirm Koch’s postulates, 10 detached leaves from B. distachyon were inoculated with a spore suspension (105 conidia/ml) of B. oryzae from a 10-day-old culture. After inoculation, the detached leaves were covered with plastic bags to maintain high relative humidity, maintained at 25°C, and monitored daily for symptom development. Leaves inoculated with sterile water served as controls. Four days after inoculation, leaves displayed symptoms similar to those observed in the field, while controls remained symptomless. The experiment was repeated. Cultures reisolated from inoculated leaves had the same morphological and molecular traits as the initial isolates. Based on the morphological characteristics and sequence analysis, the fungus was identified as B. oryzae (Krupinsky et al. 2004; Manamgoda et al. 2014). B. oryzae has previously been reported from multiple hosts including Oryza sativa, Triticum aestivum, Panicum virgatum, and Zea mays (Farr and Rossman 2017; Manamgoda et al. 2014). The current report highlights the relevance of B. distachyon as a host for B. oryzae that could be important for studies about the pathogen-host interaction in cereal crops. To our knowledge, this is the first report of B. oryzae causing leaf spot of B. distachyon in Brazil.References:Farr, D. F., and Rossman, A. Y. 2017. Fungal Databases, U. S. National Fungus Collections, ARS, USDA. Retrieved 15 November 2017 from https://nt.ars-grin.gov/fungaldatabases/ Google ScholarFitzgerald, T. L., et al. 2015. Ann. Bot. 115:717. https://doi.org/10.1093/aob/mcv010 Crossref, ISI, Google ScholarKrupinsky, J. M., et al. 2004. Can. J. Plant Pathol. 26:371. https://doi.org/10.1080/07060660409507155 Crossref, ISI, Google ScholarManamgoda, D. S., et al. 2014. Stud. Mycol. 79:221. https://doi.org/10.1016/j.simyco.2014.10.002 Crossref, ISI, Google ScholarT. E. Kaspary and C. Bellé contributed equally to this work.DetailsFiguresLiterature CitedRelated Vol. 102, No. 7 July 2018SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 27 Jun 2018Published: 26 Apr 2018First Look: 17 Jan 2018Accepted: 16 Jan 2018 Page: 1450 Information© 2018 The American Phytopathological SocietyCited byFirst Report of Bipolaris oryzae on Typha latifolia and the Pathogenicity of Its Isolates on Different Rice Varieties10 August 2022 | Acta Mycologica, Vol. 57Cochliobolus miyabeanus (brown leaf spot of rice)CABI Compendium, Vol. CABI CompendiumA polyphasic approach to delineate species in Bipolaris25 May 2020 | Fungal Diversity, Vol. 102, No. 1Bipolaris oryzae Causing Brown Leaf Spot on Echinochloa crus-galli in Southern BrazilT. E. Kaspary, C. Bellé, C. A. G. Rigon, L. Cutti, G. Casarotto, M. Gallon, and A. Merotto Junior15 March 2019 | Plant Disease, Vol. 103, No. 5
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