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Rat inferior caval vein (ICV) ligature and particular new insights with the stable gastric pentadecapeptide BPC 157

2018; Elsevier BV; Volume: 106; Linguagem: Inglês

10.1016/j.vph.2018.02.010

1879-3649

Jakša Vukojević, Marko Siroglavić, Katarina Kašnik, Tamara Kralj, Duje Stanćić, Antonio Kokot, Darko Kolarić, Domagoj Drmić, Anita Zenko Sever, Ivan Barišić, Jelena Šuran, Davor Bojić, Maša Hrelec Patrlj, Ivica Sjekavica, Katarina Horvat Pavlov, Tinka Vidović, Josipa Vlainić, Mirjana Stupnišek, Sven Seiwerth, Predrag Sikirić,

Ion Transport and Channel Regulation

Rat inferior caval vein (ICV) ligation (up to the right ovarian vein (ROV)) commonly represents a recapitulation of Virchow: with ligation leading to vessel injury, stasis, thrombosis and hemodynamic changes. We revealed that BPC 157's therapy collectively attenuated or counteracted all these events and the full syndrome. We applied BPC 157 (10 μg, 10 ng/kg) as an early regimen or as a delayed therapy. Assessment includes gross assessment by microcamera; microscopy, venography, bleeding, blood pressure, ECG, thermography, MDA and NO-level in plasma and ICV, and gene expression. Direct vein injury, thrombosis, thrombocytopenia, prolonged bleeding were all counteracted. Also, rapid presentation of collaterals and redistribution of otherwise trapped blood volume (bypassing through the left ovarian vein (LOV) and other veins), with venous hypertension, arterial hypotension and tachycardia counteraction were shown. BPC 157-rats presented raised plasma NO-values, but normal MDA-values; in ICV tissue reverted low NO-values and counteracted increased MDA-levels. Altered expression of EGR, NOS, SRF, VEGFR and KRAS in ICV, ROV and LOV revealed increased or decreased levels, while some genes continuously remained unchanged. As a new insight, BPC 157 application largely attenuated or even completely eliminated all consequences of ICV ligation in rats.