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Matrix effect of swine urine on time-resolved fluorescent nanobeads and colloidal gold immunochromatographic assay

2018; Taylor & Francis; Volume: 29; Issue: 1 Linguagem: Inglês

10.1080/09540105.2018.1439456

1465-3443

Yong Zhang, Juan Peng, Ping Guo, Guoqiang Li, Kaiyi Zhang, Xi Lv, Weihua Lai,

Receptor Mechanisms and Signaling

Immunochromatographic assay (ICA) is an efficient analytical technique and rapid, convenient, easy-to-use, low-cost, and on-site detection method that has been widely used to evaluate food safety. However, an important issue to be addressed for this method is its matrix effect. In this work, time-resolved fluorescent nanobead ICA (TRFN-ICA) and colloidal gold ICA (CG-ICA) were developed to detect clenbuterol in swine urine. Under optimized working conditions, the limits of detection of TRFN-ICA and CG-ICA were 16 and 68 pg/mL, respectively. The matrix effect on TRFN-ICA and CG-ICA was assessed in 20 swine urine samples. Results indicated that the sensitivity of TRFN-ICA was better than that of CG-ICA, and the detection time of the former was shorter than that of the latter. The matrix effect on TRFN-ICA was more serious than that on CG-ICA.