Portal de Periódicos da CAPES

MP81-20 NON-INVASIVE, DUAL-LABELING TECHNOLOGY FOR CELL TRACKING IN STEM CELL THERAPY FOR ERECTILE DYSFUNCTION

2018; Lippincott Williams & Wilkins; Volume: 199; Issue: 4S Linguagem: Inglês

10.1016/j.juro.2018.02.2728

1527-3792

Ethan Matz, Lei Dou, Fangpeng Shu, Xin Gu, Ryan Terlecki, Jennifer Z. Paxton, Ting Long, Lysette Mutkus, Frank C. Marini, James Yoo, Anthony Atala, John D. Jackson, Yuanyuan Zhang,

Tissue Engineering and Regenerative Medicine

You have accessJournal of UrologyStem Cell Research: Stem Cell Research II1 Apr 2018MP81-20 NON-INVASIVE, DUAL-LABELING TECHNOLOGY FOR CELL TRACKING IN STEM CELL THERAPY FOR ERECTILE DYSFUNCTION Ethan Matz, Lei Dou, Fangpeng Shu, Xin Gu, Ryan Terlecki, Jennifer Paxton, Ting Long, Lysette Mutkus, Frank Marini, James Yoo, Anthony Atala, John Jackson, and Yuanyuan Zhang Ethan MatzEthan Matz More articles by this author , Lei DouLei Dou More articles by this author , Fangpeng ShuFangpeng Shu More articles by this author , Xin GuXin Gu More articles by this author , Ryan TerleckiRyan Terlecki More articles by this author , Jennifer PaxtonJennifer Paxton More articles by this author , Ting LongTing Long More articles by this author , Lysette MutkusLysette Mutkus More articles by this author , Frank MariniFrank Marini More articles by this author , James YooJames Yoo More articles by this author , Anthony AtalaAnthony Atala More articles by this author , John JacksonJohn Jackson More articles by this author , and Yuanyuan ZhangYuanyuan Zhang More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2018.02.2728AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Intracavernosal administration of stem cells has demonstrated efficacy in animal models of erectile dysfunction (ED). The fate of grafted cells, however, is poorly characterized, and it is unknown if labeling technology impacts cellular function. Thus, alongside human placental stem cells (hPSC) we aim to use a novel dual-labeling technique featuring a red fluorescent protein variant mKATE and a new codon-optimized luciferase from Renilla Reniformis (mKATE-renLUC) using a lentivirus vector. We aim to determine the effect of labeling on in vitro function in preparation for future bioluminescence-tracking following intracavernosal administration. METHODS To optimize the transfection protocol, different hPSC concentrations, vectors (mKATE-renLUC, GFP-FLUC), and lentivirus titers were tested. Cell viability, proliferation, migration, and paracrine effects of hPSC expressing mKATE-renLUC were examined, and compared to non-labeled cells. Paracrine effects of labeled cells were examined using an angiogenesis array. Cell survival and distribution of hPSC expressing mKATE-renLUC were monitored by IVIS imaging system in vitro. RESULTS Cell viability, morphology, migration, and cell proliferation of mKATE-renLUC labeled hPSC were similar to those of non-labeled cells in vitro. Cell survival and migration of renLUC-labeled hPSC were efficiently monitored using IVIS imaging system. The angiogenesis array demonstrated similar expression of factors from both cell types. CONCLUSIONS This study demonstrates that dual-labeling technology using mKATE-renLUC provides a safe and effective cell tracking approach with brighter fluorophores and codon optimized luciferase. This development is promising for longer and more effective in vivo stem cell tracking after intracavernosal administration in models of ED. © 2018FiguresReferencesRelatedDetails Volume 199Issue 4SApril 2018Page: e1104 Advertisement Copyright & Permissions© 2018MetricsAuthor Information Ethan Matz More articles by this author Lei Dou More articles by this author Fangpeng Shu More articles by this author Xin Gu More articles by this author Ryan Terlecki More articles by this author Jennifer Paxton More articles by this author Ting Long More articles by this author Lysette Mutkus More articles by this author Frank Marini More articles by this author James Yoo More articles by this author Anthony Atala More articles by this author John Jackson More articles by this author Yuanyuan Zhang More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...