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Prevalence of the pathogenic crustacean virus Callinectes sapidus reovirus 1 near flow-through blue crab aquaculture in Chesapeake Bay, USA

2018; Inter-Research; Volume: 129; Issue: 2 Linguagem: Inglês

10.3354/dao03232

1616-1580

EM Flowers, AF Johnson, Robert Aguilar, EJ Schott,

Marine Bivalve and Aquaculture Studies

DAO Diseases of Aquatic Organisms Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout the JournalEditorsSpecials DAO 129:135-144 (2018) - DOI: https://doi.org/10.3354/dao03232 Prevalence of the pathogenic crustacean virus Callinectes sapidus reovirus 1 near flow-through blue crab aquaculture in Chesapeake Bay, USA Emily M. Flowers1, Andrew F. Johnson2, Robert Aguilar3, Eric J. Schott4,* 1University of Maryland School of Medicine, Baltimore, MD 21201, USA 2Marine Biology Research Division, The Scripps Institution of Oceanography University of California, San Diego, CA 92093, USA 3Smithsonian Environmental Research Center, Edgewater, MD 21037, USA 4Institute of Marine and Environmental Technology, University of Maryland Center for Environmental Science, Baltimore, MD 21202, USA *Corresponding author: schott@umces.edu ABSTRACT: Understanding the ecology of diseases is important to understanding variability in abundance, and therefore management, of marine animals exploited commercially. The blue crab Callinectes sapidus fills a crucial benthic-pelagic niche in Atlantic estuarine ecosystems and supports large commercial fisheries in both North and South America. In the USA, pre-molt blue crabs are typically held in short-term shedding (ecdysis) facilities to produce soft-shell crabs of increased value. However, mortality rates in these facilities are high and commonly associated with the pathogenic C. sapidus reovirus 1 (CsRV1). To assess whether crab mortalities in these facilities might increase CsRV1 prevalence in wild crab populations, tissue sampled from crabs collected over 2 summers either near to or far from shedding facilities using flow-through water systems were tested by reverse transcription quantitative PCR (RT-qPCR) for the presence of CsRV1 RNA. In support of our hypothesis, PCR data identified the probability of detecting CsRV1 in wild crabs sampled close to shedding facilities to be 78% higher than in crabs sampled from far sites. PCR detections were also 61-72% more probable in male crabs and 21% more likely in male and female crabs over the minimum landing size. As the prevalence at which CsRV1 was detected varied within seasons, among locations and between years, blue crab migration and/or population fluctuations appear to also be involved. KEY WORDS: Disease ecology · Callinectes sapidus · CsRV1 · Natural mortality Full text in pdf format PreviousNextCite this article as: Flowers EM, Johnson AF, Aguilar R, Schott EJ (2018) Prevalence of the pathogenic crustacean virus Callinectes sapidus reovirus 1 near flow-through blue crab aquaculture in Chesapeake Bay, USA. Dis Aquat Org 129:135-144. https://doi.org/10.3354/dao03232 Export citation RSS - Facebook - Tweet - linkedIn Cited by Published in DAO Vol. 129, No. 2. Online publication date: July 04, 2018 Print ISSN: 0177-5103; Online ISSN: 1616-1580 Copyright © 2018 Inter-Research.