FEEDBACK REGULATION OF INSULIN SECRETION BY INSULIN: ROLE OF 3′,5′-CYCLIC AMP
1970; Elsevier BV; Linguagem: Inglês
10.1016/b978-0-08-015844-0.50049-3
AutoresJ. C. Sodoyez, F. Sodoyez‐Goffaux, P. P. Foà,
Tópico(s)Diabetes Management and Research
ResumoThe insulin secretion of isolated islets of Langerhans, incubated in a medium containing glucose at the concentration of 100 mg%, was increased significantly and approximately to the same degree by glucagon (5 μg/ml), cAMP (8 mm) and DBcAMP (0.5 mm). DBcAMP was found to be a stronger insulinogenic stimulus than cAMP probably and at least in part, because it is resistant to β-cell phosphodiesterase. Bovine insulin, added to the incubation medium at the concentration of 250 μu/ml, did not affect the insulin output induced by DBcAMP, but significantly inhibited that induced by cAMP and glucagon. It is suggested that insulin decreases the level of cAMP in the β-cell by regulating the activity of phosphodiesterase and, possibly that of adenylcyclase, and that it does not interfere with the action of the nucleotide on insulin secretion.
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