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Antibacterial and Antibiofilm Activities of Psychorubrin, a Pyranonaphthoquinone Isolated From Mitracarpus frigidus (Rubiaceae)

2018; Frontiers Media; Volume: 9; Linguagem: Inglês

10.3389/fmicb.2018.00724

1664-302X

Ari S.O. Lemos, Lara Melo Campos, Lívia Melo, Maria Clara Machado Resende Guedes, Luiz Gustavo de Oliveira, Thiago P. Silva, Rossana C. N. Melo, Vinícius Novaes Rocha, Jair Adriano Kopke de Aguiar, Ana Carolina Morais Apolônio, Elita Scio, Rodrigo Luiz Fabri,

Bacterial Identification and Susceptibility Testing

Psychorubrin, a natural pyranonaphthoquinone found in different plants, has become an interesting compound in the search for new antimicrobial therapeutic agents. Here, we investigated the potential antagonistic activity of psychorubrin against planktonic and biofilm bacteria. First, psychorubrin was tested against several Gram-positive and Gram-negative bacteria strains by a broth microdilution susceptibility method. Second, bacterial killing assay, bacterial abundance, and membrane viability were evaluated. The nucleotide leakage assay was used to verify membrane destabilization while antibiofilm activities were analyzed by the effect on established biofilm, static biofilm formation, isolation of biofilm matrix assay and scanning electron microscopy. In parallel, the combinatorial effect of psychorubrin and chloramphenicol was evaluated by the checkerboard method. Psychorubrin was active against Gram-positive bacteria, showing rapid time-dependent kinetics of bacterial killing, amplified nucleotide leakage, and greater activity against the methicillin-resistant species (MRSA)