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Gas Chromatography-Mass Spectrometry (GC-MS) Analysis of Antibiotic producing Actinomycetes Soils samples

2018; Pharmascope Foundation; Volume: 9; Issue: SPL1 Linguagem: Inglês

10.26452/ijrps.v9ispl1.1317

0975-7538

Dina Mohammed Raouf AlKhafaf, Eman M. Jarallah,

Fungal Biology and Applications

In this study,a total of 120 agriculture soils samples,theirty two Actinomycetes isolates were isolated from ten different locations in Babylon city which were purified several times on International Streptomyces project-2 agar medium (ISP-2). All these isolates were tested for antibacterial activity by primary and secondary screening test against gram positive pathogenic bacteria (Staphylococcus albus,Staphylococcus aureus,and Streptococcus pyogenes) and gram negative pathogenic bacteria (Klebsillae pnemoniae,Escherichia coli,Pseudomonas aerouginosa,Seratia marcescens and Aeromonas hydrophila), televe isolates were showed antibacterial activity against these bacteria. Bacterial extraction of three of actinomycetes isolates which showed high antibacterial activity,the isolates (No.3:MS.A.21,No.8:AG.A.72,No.10:R.A.98) were chemicly characterized by gas chromotography techniques (GC-Mass) to detect volatile organic compounds.Five components were identified in the ethyl acetate bacteria extract. The compounds were Acetic acid (58.42%) as the major component followed by 2-Butanone,3-hydroxy (21.23%),Butanoic acid,2-methyl (8.76%),2, 3-Butanediol, [R- (R*, R*)] (7.75%) and last couponed propanoic acid, 2-methyl (3.84%) respectively. Figure (4-1) shows the mass spectrum and structures of the identified components.