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Mucor circinelloides : Growth, Maintenance, and Genetic Manipulation

2018; Wiley; Volume: 49; Issue: 1 Linguagem: Inglês

10.1002/cpmc.53

1934-8533

Sandeep Vellanki, María Isabel Navarro-Mendoza, Alexis Garcia, Laura Murcia, Carlos Pérez‐Arques, Victoriano Garre, Francisco E. Nicolás, Soo Chan Lee,

Microbial Natural Products and Biosynthesis

Mucor circinelloides is a fungus that belongs to the order Mucorales. It grows as mold in the environment and can cause mucormycosis, a potentially fatal infection in immunocompromised patients. M. circinelloides is a biodiesel producer and serves as a model organism for studying several biological processes, such as light responses and RNA interference-mediated gene silencing. Over the past decade, the increasing number of molecular tools has also allowed us to manipulate the genome of this fungus. This article outlines the fundamental protocols for the in vitro growth, maintenance, and genetic manipulation of M. circinelloides in the laboratory. © 2018 by John Wiley & Sons, Inc.