Impact of Bioactive Peptide Motifs on Molecular Structure, Charging, and Nonfouling Properties of Poly(ethylene oxide) Brushes
2018; American Chemical Society; Volume: 34; Issue: 21 Linguagem: Inglês
10.1021/acs.langmuir.8b00441
ISSN1520-5827
AutoresOgnen Pop‐Georgievski, Ralf Zimmermann, Ilya Kotelnikov, Vladimír Proks, Dirk Romeis, Jan Kučka, Anja Caspari, František Rypáček, Carsten Werner,
Tópico(s)Surface Modification and Superhydrophobicity
ResumoPolymer layers capable of suppressing protein adsorption from biological media while presenting extracellular matrix-derived peptide motifs offer valuable new options for biomimetic surface engineering. Herein, we provide detailed insights into physicochemical changes induced in a nonfouling poly(ethylene oxide) (PEO) brush/polydopamine (PDA) system by incorporation of adhesion ligand (RGD) peptides. Brushes with high surface chain densities (σ ≥ 0.5 chains·nm–2) and pronounced hydrophilicity (water contact angles ≤ 10°) were prepared by end-tethering of heterobifunctional PEOs (Mn ≈ 20 000 g·mol–1) to PDA-modified surfaces from a reactive melt. Using alkyne distal end group on the PEO chains, azidopentanoic-bearing peptides were coupled through a copper-catalyzed Huisgen azide–alkyne "click" cycloaddition reaction. The surface concentration of RGD was tuned from complete saturation of the PEO surface with peptides (1.7 × 105 fmol·cm–2) to values which may induce distinct differences in cell adhesion ( H3O+) from the electrolyte. Despite the distinct differences observed in the electrokinetic analysis of the surfaces bearing different amounts of RGD, it was found that the peptide presence on PEO(20 000)–PDA layers does not have a significant effect on the nonfouling properties of the system. Notably, the presented PEO(20 000)–PDA layers bearing RGD peptides in the surface concentration range 5.9 to 1.7 × 105 fmol·cm–2 reduced the protein adsorption from fetal bovine serum to less than 30 ng·cm–2, that is, values comparable to the ones obtained for pristine PEO(20 000)–PDA layers.
Referência(s)