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BIBTEX RIS

Field-deployable viral diagnostics using CRISPR-Cas13

2018; American Association for the Advancement of Science; Volume: 360; Issue: 6387 Linguagem: Inglês

10.1126/science.aas8836

ISSN

1095-9203

Autores

Cameron Myhrvold, Catherine A. Freije, Jonathan S. Gootenberg, Omar O. Abudayyeh, Hayden C. Metsky, Ann Durbin, Max J. Kellner, Amanda L. Tan, Lauren M. Paul, Leda Parham, Kimberly García, Kayla G. Barnes, Bridget Chak, Adriano Mondini, Maurício Lacerda Nogueira, Sharon Isern, Scott F. Michael, Ivette Lorenzana, Nathan L. Yozwiak, Bronwyn MacInnis, Irene Bosch, Lee Gehrke, Feng Zhang, Pardis C. Sabeti,

Tópico(s)

Cytomegalovirus and herpesvirus research

Resumo

Taking CRISPR technology further CRISPR techniques are allowing the development of technologies for nucleic acid detection (see the Perspective by Chertow). Taking advantages of the distinctive enzymatic properties of CRISPR enzymes, Gootenberg et al. developed an improved nucleic acid detection technology for multiplexed quantitative and highly sensitive detection, combined with lateral flow for visual readout. Myhrvold et al. added a sample preparation protocol to create a field-deployable viral diagnostic platform for rapid detection of specific strains of pathogens in clinical samples. Cas12a (also known as Cpf1), a type V CRISPR protein, cleaves double-stranded DNA and has been adapted for genome editing. Chen et al. discovered that Cas12a also processes single-stranded DNA threading activity. A technology platform based on this activity detected human papillomavirus in patient samples with high sensitivity. Science , this issue p. 439 , p. 444 , p. 436 ; see also p. 381

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