Cell type atlas and lineage tree of a whole complex animal by single-cell transcriptomics
2018; American Association for the Advancement of Science; Volume: 360; Issue: 6391 Linguagem: Inglês
10.1126/science.aaq1723
ISSN1095-9203
AutoresMireya Plass, Jordi Solana, F. Alexander Wolf, Salah Ayoub, Aristotelis Misios, Petar Glažar, Benedikt Obermayer, Fabian J. Theis, Christine Kocks, Nikolaus Rajewsky,
Tópico(s)Olfactory and Sensory Function Studies
ResumoMapping the planarian transcriptome A cell type's transcriptome defines the active genes that control its biology. Two groups used single-cell RNA sequencing to define the transcriptomes for essentially all cell types of a complete animal, the regenerative planarian Schmidtea mediterranea. Because pluripotent stem cells constantly differentiate to rejuvenate any part of the body of this species, all developmental lineages are active in adult animals. Fincher et al. determined the transcriptomes for most, if not all, planarian cell types, including some that were previously unknown. They also identified transition states and genes governing positional information. Plass et al. used single-cell transcriptomics and computational algorithms to reconstruct a lineage tree capturing the developmental progressions from stem to differentiated cells. They could then predict gene programs that are specifically turned on and off along the tree, and they used this approach to study how the cell types behaved during regeneration. These whole-animal transcriptome “atlases” are a powerful way to study metazoan biology. Science , this issue p. eaaq1736 , p. eaaq1723
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