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Direct evidence for α ether linkage between lignin and carbohydrates in wood cell walls

2018; Nature Portfolio; Volume: 8; Issue: 1 Linguagem: Inglês

10.1038/s41598-018-24328-9

2045-2322

Hiroshi Nishimura, Akihiro Kamiya, Takashi Nagata, Masato Katahira, Takashi Watanabe,

Horticultural and Viticultural Research

Cross-linking between lignin and polysaccharide in plant cell-wall determines physical, chemical, and biological features of lignocellulosic biomass. Since Erdmann's first report in 1866, numerous studies have suggested the presence of a bond between hemicelluloses and lignin; however, no clear evidence for this interaction has been reported. We describe the first direct proof of covalent bonding between plant cell-wall polysaccharides and lignin. Nuclear magnetic resonance spectroscopy was used to observe the long-range correlations through an α-ether bond between lignin and the primary hydroxyl group of a mannose residue in glucomannan. Complete signal assignment of the cognate structural units was also achieved. Thus, we identified lignin-carbohydrate bonds by complete connectivity analysis from the phenylpropane unit to the carbohydrate moiety.