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CRISPOR: intuitive guide selection for CRISPR/Cas9 genome editing experiments and screens

2018; Oxford University Press; Volume: 46; Issue: W1 Linguagem: Inglês

10.1093/nar/gky354

1362-4962

Jean‐Paul Concordet, Maximilian Haeussler,

Evolution and Genetic Dynamics

CRISPOR.org is a web tool for genome editing experiments with the CRISPR–Cas9 system. It finds guide RNAs in an input sequence and ranks them according to different scores that evaluate potential off-targets in the genome of interest and predict on-target activity. The list of genomes is continuously expanded, with more 150 genomes added in the last two years. CRISPOR tries to provide a comprehensive solution from selection, cloning and expression of guide RNA as well as providing primers needed for testing guide activity and potential off-targets. Recent developments include batch design for genome-wide CRISPR and saturation screens, creating custom oligonucleotides for guide cloning and the design of next generation sequencing primers to test for off-target mutations. CRISPOR is available from http://crispor.org, including the full source code of the website and a stand-alone, command-line version.