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Phytochemical screening, total phenolic content, antioxidant, and cytotoxic activity of five peruvian plants on human tumor cell lines

2018; Medknow; Volume: 10; Issue: 2 Linguagem: Inglês

10.4103/pr.pr_109_17

0976-4836

Oscar Herrera-Calderón, Corina Alvarado-Puray, JorgeLuis Arroyo-Acevedo, Juan Pedro Rojas-Armas, Víctor Chumpitaz-Cerrate, Renán Dilton Hañari-Quispe, Rita Valenzuela-Herrera,

Phytochemicals and Medicinal Plants

Pharmacognosy Research,2018,10,2,161-165.DOI:10.4103/pr.pr_109_17Published:April 2018Type:Original ArticleAuthors:Oscar Herrera‑Calderon, Corina Alvarado‑Puray, Jorge Luis Arroyo‑Acevedo, Juan Pedro Rojas‑Armas, Victor Chumpitaz‑Cerrate, Renán Hañari‑Quispe, and Rita Valenzuela‑Herrera Author(s) affiliations:Oscar Herrera-Calderon1, Corina Alvarado-Puray2, Jorge Luis Arroyo-Acevedo3, Juan Pedro Rojas-Armas3, Victor Chumpitaz-Cerrate4, Renán Hañari-Quispe5, Rita Valenzuela-Herrera6 1Laboratory of Pharmacognosy, Faculty of Pharmacy and Biochemistry, Universidad Nacional Mayor de San Marcos, PERU. 2Department of Pharmacy, Hospital Carlos Lanfranco La Hoz, PERU. 3Academic Department of Dynamic Sciences, Laboratory of Experimental Pharmacology, Faculty of Medicine, Universidad Nacional Mayor de San Marcos, PERU. 4Laboratory of Physiology and Pharmacology, Faculty of Dentistry, Universidad Nacional Mayor de San Marcos, Lima, PERU. 5Laboratory of Animal Physiology, Universidad Andina Néstor Cáceres Velasquez, Puno, Peru 6 Faculty of Pharmacy and Biochemistry, Universidad Nacional San Luis Gonzaga de Ica, Ica,PERU. Abstract:Background: Cancer is considered a serious public health problem in the world; searching alternative treatments of medicinal plants constitute a promissory field to find new anticancer drugs. Objective: The aim of this study is to evaluate the phytochemical screening, total phenolic content (TPC), antioxidant, and cytotoxic activity of ethanol extracts of Waltheria ovata (WO), Piper aduncum (PA), Myrciaria dubia (MD), Physalis peruviana (PP), and Geranium dielsianum (GD) on human tumor cell lines. Materials and Methods: Phytochemical screening was assessed using chemical reactives. TPC was developed using Folin Ciocalteu reactive, and the antioxidant activity was determined against 2,2‑diphenyl‑1‑picrylhydrazyl (DPPH), and 2,2‑azino‑bis (3‑ethylbenzothiazoline‑6‑sulfonic acid) (ABTS) radicals by spectrophotometry. The cytotoxic activity was determined on human tumor cell lines followed as: MCF‑7, H‑460, HT‑29, M‑14, K‑562, and DU‑145. Results: Phytochemical study confirmed flavonoids and phenolic compounds in all extracts. TPC was found to be the highest in WO extract (1250 mg of gallic acid equivalent/g of dried extract) rather than other extracts. The highest antioxidant activity was stablished in WO extract for DPPH and ABTS radical scavenging tests (inhibitory concentration [IC50] = 0.89 ± 0.01 μg/mL, IC50 = 4.20 ± 1.50 μg/mL). Ethanolic extracts (μg/mL) showed low cytotoxicity on human tumor cell lines (CI50 >20 μg/mL) for PA, PP, GD, MD. Meanwhile, WO presented cytotoxicity on H460, MCF‑7, and K562 tumor cell lines. Conclusion: In our findings, WO and MD extracts revealed a high antioxidant and TPC. WO exhibited better cytotoxic effect compared with 5‑FU. Hence, these medicinal plants could be effective to prevent cancer disease and oxidative stress. Keywords:Antioxidant activity, Cancer, cytotoxicity, Oxidative stress, PhytochemicalView:PDF (1.45 MB)