Produção Nacional
Revisado por pares
Multi-parameter approach to evaluate the timing of memory status after 17DD-YF primary vaccination
2018; Public Library of Science; Volume: 12; Issue: 6 Linguagem: Inglês
10.1371/journal.pntd.0006462
ISSN1935-2735
AutoresChristiane Costa-Pereira, Ana Carolina Campi‐Azevedo, Jordana Grazziela Coelho-dos-Reis, Vanessa Peruhype-Magalhães, Márcio Sobreira Silva Araújo, Lis Ribeiro do Valle Antonelli, Cristina Toscano Fonseca, Jandira Aparecida Lemos, Luiz Cosme Cote Malaquias, Matheus de Souza Gomes, Laurence Rodrigues do Amaral, María Rios, Caren Chancey, Harold Richard Persi, Jorge Marcelo Pereira, Maria de Lourdes de Sousa Maia, Marcos da Silva Freire, Reinaldo de Menezes Martins, Akira Homma, Marisol Simões, Anna Yoshida Yamamura, Roberto Farias, Alessandro Pecego Martins Romano, Carla Magda Allan Santos Domingues, Pedro Luíz Tauil, Pedro Fernando da Costa Vasconcelos, Iramaya Rodrigues Caldas, Luiz Antônio Bastos Camacho, Andréa Teixeira−Carvalho, Olindo Assis Martins‐Filho,
Tópico(s)Malaria Research and Control
ResumoIn this investigation, machine-enhanced techniques were applied to bring about scientific insights to identify a minimum set of phenotypic/functional memory-related biomarkers for post-vaccination follow-up upon yellow fever (YF) vaccination. For this purpose, memory status of circulating T-cells (Naïve/early-effector/Central-Memory/Effector-Memory) and B-cells (Naïve/non-Classical-Memory/Classical-Memory) along with the cytokine profile (IFN/TNF/IL-5/IL-10) were monitored before-NV(day0) and at distinct time-points after 17DD-YF primary vaccination-PV(day30-45); PV(year1-9) and PV(year10-11). A set of biomarkers (eEfCD4; EMCD4; CMCD19; EMCD8; IFNCD4; IL-5CD8; TNFCD4; IFNCD8; TNFCD8; IL-5CD19; IL-5CD4) were observed in PV(day30-45), but not in NV(day0), with most of them still observed in PV(year1-9). Deficiencies of phenotypic/functional biomarkers were observed in NV(day0), while total lack of memory-related attributes was observed in PV(year10-11), regardless of the age at primary vaccination. Venn-diagram analysis pre-selected 10 attributes (eEfCD4, EMCD4, CMCD19, EMCD8, IFNCD4, IL-5CD8, TNFCD4, IFNCD8, TNFCD8 and IL-5CD4), of which the overall mean presented moderate accuracy to discriminate PV(day30-45)&PV(year1-9) from NV(day0)&PV(year10-11). Multi-parameter approaches and decision-tree algorithms defined the EMCD8 and IL-5CD4 attributes as the top-two predictors with moderated performance. Together with the PRNT titers, the top-two biomarkers led to a resultant memory status observed in 80% and 51% of volunteers in PV(day30-45) and PV(year1-9), contrasting with 0% and 29% found in NV(day0) and PV(year10-11), respectively. The deficiency of memory-related attributes observed at PV(year10-11) underscores the conspicuous time-dependent decrease of resultant memory following17DD-YF primary vaccination that could be useful to monitor potential correlates of protection in areas under risk of YF transmission.
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