Produção Nacional
Revisado por pares
First Report of Yerba Mate Wilt Caused by Ceratocystis fimbriata in Brazil
2018; American Phytopathological Society; Volume: 102; Issue: 11 Linguagem: Inglês
10.1094/pdis-02-18-0228-pdn
ISSN1943-7692
AutoresA. F. dos Santos, Maria Alves Ferreira, Celso Garcia Auer, C Bührer, Nícolas Manarim de Brito, R. M. Scremin, Maria Cecília Mireski,
Tópico(s)Fermentation and Sensory Analysis
ResumoHomePlant DiseaseVol. 102, No. 11First Report of Yerba Mate Wilt Caused by Ceratocystis fimbriata in Brazil PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Yerba Mate Wilt Caused by Ceratocystis fimbriata in BrazilA. F. dos Santos, M. A. Ferreira, C. G. Auer, C. B. Buhrer, N. M. Brito, R. M. Scremin, and M. C. MireskiA. F. dos Santos, M. A. Ferreira†Corresponding author: M. A. Ferreira; E-mail: E-mail Address: mariaferreira@dfp.ufla.brhttp://orcid.org/0000-0001-9401-7142, C. G. Auer, C. B. Buhrer, N. M. Brito, R. M. Scremin, and M. C. MireskiAffiliationsAuthors and Affiliations A. F. dos Santos , Embrapa Florestas, Colombo, Paraná, CEP 83411-000, Brazil M. A. Ferreira † , Universidade Federal de Lavras, Lavras, Minas Gerais, CEP 37200-000 Brazil C. G. Auer C. B. Buhrer , Embrapa Florestas, Colombo, Paraná, CEP 83411-000, Brazil N. M. Brito , Universidade Federal do Paraná, Curitiba, Paraná, Brazil R. M. Scremin , Instituto Marcos Enriette, Curitiba, Paraná, Brazil M. C. Mireski , Universidade Federal do Paraná, Curitiba, Paraná, Brazil. Published Online:4 Sep 2018https://doi.org/10.1094/PDIS-02-18-0228-PDNAboutSections ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Yerba mate (Ilex paraguariensis St. Hil.) is a native South American tree species cultivated in Argentina, Uruguay, Brazil, and Paraguay and used to prepare a caffeine-rich infused drink called mate or chimarrão. Since 2016, up to 5% mortality of plants has been reported in commercial plantations at São Mateus do Sul and Arvorezinha in the states of Paraná (PR) and Rio Grande do Sul (RS), respectively. The death of the plants was noticed in plants of different ages after wilting of the leaves. Stem cross sections of symptomatic plants showed radial darkening. Fungal isolates were collected from internal darkened stem-base sections and grown in potato dextrose agar medium (PDA). The isolates were characterized using DNA sequencing and morphological analysis. Ten fungal colonies of a Ceratocystis sp. were obtained and deposited in the fungal collection of Embrapa Florestas, Colombo (PR), Brazil. These isolates produced ascomatal bases that were submerged in the agar. Bases were globose, dark, 110 to 218 µm wide, 108 to 582 µm long, and had straight necks terminating in ostiolar hyphae that were divergent, hyaline, and 18.5 to 55 µm long. Asci were not seen. Ascospores were hyaline and ovoid, with a hat-shaped 3.0 to 5.0 × 2.0 to 4.0 µm. Endoconidiophores were wider at the base and tapered toward the apex; were 20 to 59 µm long and 3 to 6 µm wide at the apex; and produced cylindrical, straight, hyaline to pale brown, aseptate conidia (8.5 to 18 × 2 to 3.7 µm). Endoconidiophore-producing doliform conidia were not seen. All isolates produced dark brown, 9 to 12 × 6 to 10 µm aleurioconidia. The internal transcribed spacer (ITS) region of ribosomal DNA and the mating type locus (MAT1-1-2) were sequenced using polymerase chain reaction products from three isolates (C1, C4, and C8) according to Ferreira et al. (2017). Phylogenetic trees were generated using maximum parsimony. Phylogenetic analyses of the DNA sequences of the ITS (accession no. MH221142) and MAT1-1-2 (MH221143) grouped the yerba mate isolates together with PM20 with 75 and 98% bootstrap support, respectively. Intraspecific relationships among Ceratocystis fimbriata ITS showed that three isolates of this study belonged to the Latin American Clade and corresponded to the PM20 MAT1-1-2 sequence (MF347680). The fungus was first reported in RS in 2003 in Acacia mearnsii (dos Santos and Ferreira 2003). Pathogenicity of two isolates was evaluated by inoculating (incision) the stem of 10, 6-month-old plants 2 cm above the soil surface. A PDA disk with active mycelium (12 days old) was inserted (between the bark and the wood) and the incision covered with tape. The control consisted of plants inoculated with a sterile PDA disk without fungal mycelium. The material was kept in a greenhouse for 60 days. The presence or absence of wilting symptoms was recorded during the experiment. The experiment was conducted twice. The plants started to wilt at 14 days after inoculation (DAI), when 10% of the plants showed symptoms; then, at 60 DAI, all inoculated plants were dead. C. fimbriata was reisolated from infected plants, fulfilling Koch's postulates. This finding contributes to understanding the diversity of this pathogen in the region where other hosts occur, such as kiwifruit. It also confirms the presence of cryptic species in RS. However, host range and interfertility studies are needed to reveal new species (Harrington et al. 2014). This is the first report of C. fimbriata causing wilt in yerba mate in the world.References:Ferreira, M. A., et al. 2017. Trop. Plant Pathol. 42:86. https://doi.org/10.1007/s40858-017-0131-y Crossref, ISI, Google ScholarHarrington, T. C., et al. 2014. Mycologia 106:224. https://doi.org/10.3852/13-189 Crossref, ISI, Google Scholardos Santos, A. F., and Ferreira, F. A. 2003. Fitopatol. Bras. 28:325. https://doi.org/10.1590/S0100-41582003000300020 Crossref, Google ScholarFunding: Funding was provided by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Conselho Nacional de Desenvolvimento Científico e Tecnológico, and Fundação de Amparo à Pesquisa do Estado de Minas Gerais.DetailsFiguresLiterature CitedRelated Vol. 102, No. 11 November 2018SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 25 Oct 2018Published: 4 Sep 2018First Look: 30 May 2018Accepted: 23 May 2018 Pages: 2381-2381 Information© 2018 The American Phytopathological SocietyFundingCoordenação de Aperfeiçoamento de Pessoal de Nível SuperiorConselho Nacional de Desenvolvimento Científico e TecnológicoFundação de Amparo à Pesquisa do Estado de Minas GeraisCited byCeratocystis fimbriata (Ceratocystis blight)CABI Compendium, Vol. CABI CompendiumAggressiveness and screening for host responses in yerba mate ( Ilex paraguariensis ) clones to a wilt disease caused by Ceratocystis fimbriata12 March 2021 | Forest Pathology, Vol. 51, No. 3Morphophysiological characterization of Ceratocystis fimbriata isolates from yerba mate1 January 2021 | Ciência Rural, Vol. 51, No. 3Ceratocystis fimbriata Employs a Unique Infection Strategy Targeting Peltate Glandular Trichomes of Sweetpotato (Ipomoea batatas) PlantsYong Sun, Mengqiu Li, Yansu Wang, Lianwei Li, Meng Wang, Xintong Li, Mengke Xu, Gary J. Loake, Ming Guo, and Jihong Jiang4 November 2020 | Phytopathology®, Vol. 110, No. 12
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