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Efficient detection of Zika virus RNA in patients’ blood from the 2016 outbreak in Campinas, Brazil

2018; Nature Portfolio; Volume: 8; Issue: 1 Linguagem: Inglês

10.1038/s41598-018-22159-2

2045-2322

Carla C. Judice, Jeslin J. L. Tan, Pierina Lorencini Parise, Yiu‐Wing Kam, Guilherme Paier Milanez, Juliana Almeida Leite, Leonardo C. Caserta, Clarice Weis Arns, Mariângela Ribeiro Resende, Rodrigo Nogueira Angerami, Eliana Amaral, Renato Passini, André Ricardo Ribas Freitas, Fábio Trindade Maranhão Costa, José Luiz Proença-Módena, Lisa F. P. Ng, Gláucia María Pastore, Helaine Maria Besteti Pires Mayer-Milanez, Carolina C. Ribeiro‐do‐Valle, Roseli Calil, Maria Laura Costa, João Renato Bennini, Giuliane Jesus Lajos, Márcia Teixeira Garcia, Kleber Yotsumoto Fertrin, Maria Luíza Moretti, Marcos Tadeu Nolasco da Silva, Ana Carolina Coan, Maria Francisca Colella‐Santos, Andrea Paula Bruno von Zuben, Marco Aurélio Ramirez Vinolo, Rodrigo Ramos Catharino, Gabriela Mansano do Nascimento, Matheus Cavalheiro Martini, Ana Paula Moraes, Ana Lucia Rodrigues Soledade, Daniel A. Toledo-Teixeira, Évellyn Ribeiro de Morais, Felipe Rebelo Santos, Monique Fontana,

Malaria Research and Control

Infection with Zika virus (ZIKV), a mosquito-borne flavivirus has been casually linked with increased congenital microcephaly in Brazil from 2015 through 2016. Sensitive and specific diagnosis of patients with Zika fever (ZIKF) remains critical for patient management. We developed a ZIKV NS5 qRT-PCR assay by combining primers described by Balm et al. and a new Taqman probe. The assay was evaluated and compared with another assay described by Lanciotti et al. (ZIKV 1107) using 51 blood and 42 urine samples from 54 suspected ZIKV patients. ZIKV NS5 performed better in terms of sensitivity with more samples detected as ZIKV-positive (n = 37) than ZIKV 1107 (n = 34) for urine, and ZIKV-positive (n = 29) than ZIKV 1107 (n = 26) for blood. Both assays displayed good overall agreement for urine (κappa = 0.770) and blood (κappa = 0.825) samples. Improved availability of validated diagnostic tests, such ZIKV NS5 qRT-PCR, will be critical to ensure adequate and accurate ZIKV diagnosis.