Produção Nacional
Revisado por pares
First World Report of Cucurbit Aphid-Borne Yellows Virus Infecting Passionfruit
2018; American Phytopathological Society; Volume: 102; Issue: 12 Linguagem: Inglês
10.1094/pdis-04-18-0694-pdn
ISSN1943-7692
AutoresAndreza Henrique Vidal, Márcio Martinello Sanches, D. M. T. Alves-Freitas, E. F. M. Abreu, Cristiano Lacorte, Bruna Pinheiro-Lima, Raul Castro Carriello Rosa, O. N. de Jesus, Magnólia A. Campos, Arvind Varsani, Simone G. Ribeiro,
Tópico(s)CRISPR and Genetic Engineering
ResumoHomePlant DiseaseVol. 102, No. 12First World Report of Cucurbit Aphid-Borne Yellows Virus Infecting Passionfruit PreviousNext DISEASE NOTES OPENOpen Access licenseFirst World Report of Cucurbit Aphid-Borne Yellows Virus Infecting PassionfruitA. H. Vidal, M. M. Sanches, D. M. T. Alves-Freitas, E. F. M. Abreu, C. Lacorte, B. Pinheiro-Lima, R. C. C. Rosa, O. N. Jesus, M. A. Campos, A. Varsani, and S. G. RibeiroA. H. Vidal, M. M. Sanches, D. M. T. Alves-Freitas, E. F. M. Abreuhttp://orcid.org/0000-0002-0792-5183, C. Lacorte, B. Pinheiro-Lima, R. C. C. Rosa, O. N. Jesus, M. A. Campos, A. Varsani, and S. G. Ribeiro†Corresponding author: S. G. Ribeiro; E-mail: E-mail Address: simone.ribeiro@embrapa.brhttp://orcid.org/0000-0002-4965-1424AffiliationsAuthors and Affiliations A. H. Vidal , Embrapa Recursos Genéticos e Biotecnologia, Brasília, DF, Brazil, and Universidade Federal de Campina Grande, Cuité, PB, Brazil M. M. Sanches D. M. T. Alves-Freitas E. F. M. Abreu C. Lacorte B. Pinheiro-Lima , Embrapa Recursos Genéticos e Biotecnologia, Brasília, DF, Brazil R. C. C. Rosa , Embrapa Agrobiologia, Seropédica, RJ, Brazil O. N. Jesus , Embrapa Mandioca e Fruticultura, Cruz das Almas, BA, Brazil M. A. Campos , Universidade Federal de Campina Grande, Cuité, PB, Brazil A. Varsani , The Biodesign Center for Fundamental and Applied Microbiomics, Center of Evolution and Medicine, School of Life Sciences, Arizona State University, Tempe S. G. Ribeiro † , Embrapa Recursos Genéticos e Biotecnologia, Brasília, DF, Brazil. Published Online:24 Sep 2018https://doi.org/10.1094/PDIS-04-18-0694-PDNAboutSections ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Passionfruit (Passiflora spp.) is a tropical fruit used for commercial fruit juice production. Passionfruit woodiness caused by cowpea aphid-borne mosaic virus (CABMV, genus Potyvirus, family Potyviridae) is the most damaging and widespread viral disease affecting passionfruit in Brazil (Nascimento et al. 2006). In recent years, high incidence and severity of virus-like symptoms, such as blisters, mosaic, and leaf deformation, were reported by passionfruit growers in Bahia, the main passionfruit producing state in Brazil. Symptomatic leaves of passionfruit were collected in Lençóis (P. edulis, n = 14; P. alata, n = 3; and P. cincinnata, n = 4) and Jussiape (P. edulis, n = 9), Bahia State, in 2015. Double-stranded RNA was extracted from these 21 samples, pooled, and sequenced in an Illumina HiSeq 2500 sequencer (Macrogen, South Korea). De novo assembly of the reads using the ABYSS 1.9 program with K-mer = 64 resulted in 22 contigs corresponding to CABMV. Moreover, five contigs with high nucleotide identity (85 to 98%) with sequences of cucurbit aphid-borne yellows virus (CABYV) (genus Polerovirus, family Luteoviridae) were also identified. A CABYV sequence (JF939814.1) was used as a reference to map the short reads using Geneious 9.1, and 33,290 reads were mapped, resulting in a consensus contig of 5,643 nt comprising a nearly complete virus genome (CABYV-PF, MH257573). The CABYV-PF sequence shares 87 to 94% nt identities with the genome sequences of several CABYV isolates. To validate the high-throughput sequencing results, the primers CABYV_377F (5′-GATACAACGAAACCAGACAAACCTT-3′) and CABYV_1186R (5′-TTGCTTAACACAGATGCATGAGTTT-3′) covering genes P0/P1 of CABYV-PF were designed. These and the primers CE-9 and CE-10, which amplify the CP/MP genes (Kassem et al. 2013), were used to recover CABYV segments from the passionfruit pooled RNA. Amplicons of the expected size of 795 and 598 nt were obtained, cloned into pJET1.2 (ThermoFisher, U.S.A.), and Sanger sequenced. Sequences of two clones from CP/MP shared 100% identity with the CABYV-PF sequence and had the highest identity (99%) with a CABYV isolate from Italy (EF029113.1). Sequences of two clones corresponding to P0/P1 showed 97 to 100% identity with CABYV-PF and shared 95 to 96% identity with a CABYV isolate from melon collected in Brazil (LC217994.1). To assess the incidence of CABYV in all samples, reverse transcription polymerase chain reaction (RT-PCR) was performed with primers CE-9/CE-10 in RNA extracted from individual plants. After electrophoresis, Southern blot analysis was carried out. The gel was blotted to a nylon membrane that was hybridized with a CABYV-CP/MP-derived 32P-labeled probe. Presence of CABMV was evaluated by RT-PCR with primers CABMVM1MX_3726F/CABMVM1MX_5039R (Fontenele et al. 2018). The incidence of CABYV in plants from Lençóis was 52%. The three sampled Passiflora species were found to be infected with CABMV (eight P. edulis, two P. alata, and one P. cincinnata). CABMV was detected in 91% of the plants, and mixed infection with CABYV and CABMV was identified in 48% of the plants. The CABYV and CABMV infection frequencies were lower in Jussiape (11 and 55%, respectively), and no mixed infection was detected. CABYV has a wide host range and infects cucurbits and weeds from Brassicaceae, Lamiaceae, and Asteraceae in many countries. However, this is the first record of CABYV in passionfruit in the world. CABYV and CABMV are vectored by Aphis gossypii and Myzus persicae, and the more severe symptoms observed in the field may be owing to mixed infections. Studies are in progress to characterize CABYV isolates from passionfruit and to determine its distribution, alternative hosts, and interactions with CABMV.References:Fontenele, R. S., et al. 2018. Viruses 10:169. https://doi.org/10.3390/v10040169 Crossref, ISI, Google ScholarKassem, M. A., et al. 2013. Phytopathology 103:1188. https://doi.org/10.1094/PHYTO-11-12-0280-R Link, ISI, Google ScholarNascimento, A. V. S., et al. 2006. Arch. Virol. 151:1797. https://doi.org/10.1007/s00705-006-0755-6 Crossref, ISI, Google ScholarFunding: This study was supported by grants from Empresa Brasileira de Pesquisa Agropecuária (Embrapa), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), and Fundação de Apoio à Pesquisa do Distrito Federal (FAPDF). A. H. Vidal is supported by a scholarship form CAPES. D. M. T. Alves-Freitas and B. Pinheiro-Lima are recipients of fellowships from CNPq.DetailsFiguresLiterature CitedRelated Vol. 102, No. 12 December 2018SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 20 Nov 2018Published: 24 Sep 2018First Look: 5 Jun 2018Accepted: 2 Jun 2018 Pages: 2665-2665 Information© 2018 The American Phytopathological SocietyFundingEmpresa Brasileira de Pesquisa AgropecuáriaConselho Nacional de Desenvolvimento Científico e TecnológicoFundação de Apoio à Pesquisa do Distrito FederalCited byOccurrence, Distribution, and Management of Aphid-Transmitted Viruses in Cucurbits in Spain7 March 2023 | Pathogens, Vol. 12, No. 3Construction of an infectious full-length cDNA clone of a recombinant isolate of cucurbit aphid-borne yellows virus from Brazil28 October 2022 | Virus Genes, Vol. 59, No. 1Characterization of Cucurbit Aphid-Borne Yellows Virus (CABYV) from Passion Fruit in Brazil: Evidence of a Complex of Species within CABYV Isolates1 February 2023 | Viruses, Vol. 15, No. 2First Report of Cucurbit Aphid-Borne Yellows Virus Infecting Groundnut in KenyaAnthony S. Mabele, Francis N. Muyekho, and Hassan K. Were4 January 2023 | Plant Health Progress, Vol. 0, No. 0First Report of Cucurbit Aphid-Borne Yellows Virus Causing Yellowing Disease on Pumpkin (Cucurbita pepo) and Cucumber (Cucumis sativus) in BulgariaV. Radeva-Ivanova, G. Pasev, R. Lyall, M. Angelov, A. N. Nankar, and D. Kostova-Protochristova1 August 2022 | Plant Disease, Vol. 106, No. 9Genetic gains in Passiflora for resistance to cowpea aphid-borne mosaic virus using recurrent selection26 August 2022 | Euphytica, Vol. 218, No. 9Detección molecular de virus en cultivos, plántulas y semillas de gulupa (Passiflora edulis f. edulis) en el oriente de Antioquia1 May 2022 | Bioagro, Vol. 34, No. 2Virome analysis for identification of viruses associated with asymptomatic infection of purple passion fruit ( Passiflora edulis f. edulis) in Colombia14 September 2021 | The Journal of Horticultural Science and Biotechnology, Vol. 97, No. 2Cucurbit aphid-borne yellows virus (Cucurbit aphid-borne yellows)CABI Compendium, Vol. CABI CompendiumCowpea aphid-borne mosaic virusCABI Compendium, Vol. CABI CompendiumHow segregating populations of passion fruit react to CABMV infection?21 April 2021 | European Journal of Plant Pathology, Vol. 160, No. 4Occurrence of lettuce chlorosis virus in Passiflora spp. in Brazil15 March 2021 | Journal of Plant Pathology, Vol. 103, No. 2Development of polyclonal antisera against movement proteins from three poleroviruses infecting cucurbits2 September 2020 | Phytopathology Research, Vol. 2, No. 1The recombinant isolate of cucurbit aphid‐borne yellows virus from Brazil is a polerovirus transmitted by whiteflies23 April 2020 | Plant Pathology, Vol. 69, No. 6First Report of Cucurbit Aphid-Borne Yellows Virus in Passion Fruit Plants Exhibiting Mosaic and Mottling in ChinaShao-kang Zhang, Tian-yu Zhao, Jin-tao Liu, Liang-yu Zi, Xiao-yu Li, Ying Wang, Zong-ying Zhang, Da-wei Li, Jia-lin Yu, and Cheng-gui Han4 December 2019 | Plant Disease, Vol. 104, No. 2Complete genome sequence of a Passion fruit yellow mosaic virus (PFYMV) isolate infecting purple passionfruit (Passiflora edulis f. edulis)1 January 2019 | Revista Facultad Nacional de Agronomía Medellín, Vol. 72, No. 1
Referência(s)