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Ultrafast neuronal imaging of dopamine dynamics with designed genetically encoded sensors

2018; American Association for the Advancement of Science; Volume: 360; Issue: 6396 Linguagem: Inglês

10.1126/science.aat4422

1095-9203

Tommaso Patriarchi, Jounhong Ryan Cho, Katharina Merten, Mark W. Howe, Aaron Marley, Wei-Hong Xiong, Robert Folk, Gerard Joey Broussard, Ruqiang Liang, Min Jee Jang, Haining Zhong, Daniel A. Dombeck, Mark von Zastrow, Axel Nimmerjahn, Viviana Gradinaru, John T. Williams, Lin Tian,

Advanced Fluorescence Microscopy Techniques

Imaging dopamine release in the brain Neuromodulator release alters the function of target circuits in poorly known ways. An essential step to address this knowledge gap is to measure the dynamics of neuromodulatory signals while simultaneously manipulating the elements of the target circuit during behavior. Patriarchi et al. developed fluorescent protein–based dopamine indicators to visualize spatial and temporal release of dopamine directly with high fidelity and resolution. In the cortex, two-photon imaging with these indicators was used to map dopamine activity at cellular resolution. Science , this issue p. eaat4422