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Knockdown of KIF26B inhibits breast cancer cell proliferation, migration, and invasion

2018; Dove Medical Press; Volume: Volume 11; Linguagem: Inglês

10.2147/ott.s163346

1178-6930

Shudong Gu, Haibin Liang, Donghui Qi, Liyan Mao, Guoxin Mao, Qian Li, Shu Zhang,

Genomics and Chromatin Dynamics

Knockdown of KIF26B inhibits breast cancer cell proliferation, migration, and invasion Shudong Gu,1,* Haibin Liang,2,* Donghui Qi,3 Liyan Mao,4 Guoxin Mao,1 Li Qian,1 Shu Zhang5 1Department of Oncology, Affiliated Hospital of Nantong University, Nantong 226001, China; 2Department of General Surgery, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China; 3Medical College of Nantong University, Nantong 226001, China; 4Department of Endoscopic Diagnosis and Treatment of Digestive Diseases, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China; 5Department of Pathology, Affiliated Hospital of Nantong University, Nantong 226001, China *These authors contributed equally to this work Background: Kinesin family member 26B (KIF26B) plays a key role in the development and progression of many human cancers. However, the role and underlying mechanisms of KIF26B in breast cancer cells remain unknown. Materials and methods: In this study, we inhibited the expression of KIF26B in MDA-MB-231 and MCF-7 cells using lentivirus-delivered shRNA. Results: Lentivirus-mediated KIF26B knockdown significantly suppressed cell proliferation, colony formation, migration, and invasion. Furthermore, cell cycle analyses revealed that the percentage of cells in the G0/G1 phase was significantly increased in KIF26B knockdown cells. Moreover, the knockdown of KIF26B significantly promoted cell apoptosis via the upregulation of cleaved caspase-3 and Bax. Conclusion: Our data indicate that KIF26B plays a pivotal role in tumor growth and metastasis in breast cancer cells and may be a potential therapeutic target for treating breast cancer. Keywords: KIF26B, breast cancer cell, proliferation, migration, invasion