Portal de Periódicos da CAPES

Temporal window for detection of inflammatory disease using dynamic cell tracking with time-lapse MRI

2018; Nature Portfolio; Volume: 8; Issue: 1 Linguagem: Inglês

10.1038/s41598-018-27879-z

2045-2322

Max Masthoff, Sandra Gran, Xueli Zhang, Lydia Wachsmuth, Michael Bietenbeck, Anne Helfen, Walter Heindel, Lydia Sorokin, Johannes Roth, Michel Eisenblätter, Moritz Wildgruber, Cornelius Faber,

Barrier Structure and Function Studies

Abstract Time-lapse MRI was implemented for dynamic non-invasive cell tracking of individual slowly moving intravascular immune cells. Repetitive MRI acquisition enabled dynamic observation of iron oxide nanoparticle (ION) labelled cells. Simulations of MRI contrast indicated that only cells moving slower than 1 µm/s were detectable. Time-lapse MRI of the brain was performed after either IONs or ION-labelled monocytes were injected intravenously into naïve and experimental autoimmune encephalomyelitis (EAE) bearing mice at a presymptomatic or symptomatic stage. EAE mice showed a reduced number of slow moving, i.e. patrolling cells before and after onset of symptoms as compared to naïve controls. This observation is consistent with the notion of altered cell dynamics, i.e. higher velocities of immune cells rolling along the endothelium in the inflamed condition. Thus, time-lapse MRI enables for assessing immune cell dynamics non-invasively in deep tissue and may serve as a tool for detection or monitoring of an inflammatory response.