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The product of the c-ets-1 proto-oncogene and the related Ets2 protein act as transcriptional activators of the long terminal repeat of human T cell leukemia virus HTLV-1.

1990; Springer Nature; Volume: 9; Issue: 10 Linguagem: Inglês

10.1002/j.1460-2075.1990.tb07511.x

1460-2075

Rémy Bosselut, Janet F. Duvall, Anne Gégonne, M Bailly, Agnès Hémar, John Brady, Jacques Ghysdael,

Vector-Borne Animal Diseases

Research Article1 October 1990free access The product of the c-ets-1 proto-oncogene and the related Ets2 protein act as transcriptional activators of the long terminal repeat of human T cell leukemia virus HTLV-1. R. Bosselut R. Bosselut INSERM U186/CNRS URA 1160 Institut Pasteur, Lille, France. Search for more papers by this author J. F. Duvall J. F. Duvall INSERM U186/CNRS URA 1160 Institut Pasteur, Lille, France. Search for more papers by this author A. Gégonne A. Gégonne INSERM U186/CNRS URA 1160 Institut Pasteur, Lille, France. Search for more papers by this author M. Bailly M. Bailly INSERM U186/CNRS URA 1160 Institut Pasteur, Lille, France. Search for more papers by this author A. Hémar A. Hémar INSERM U186/CNRS URA 1160 Institut Pasteur, Lille, France. Search for more papers by this author J. Brady J. Brady INSERM U186/CNRS URA 1160 Institut Pasteur, Lille, France. Search for more papers by this author J. Ghysdael J. Ghysdael INSERM U186/CNRS URA 1160 Institut Pasteur, Lille, France. Search for more papers by this author R. Bosselut R. Bosselut INSERM U186/CNRS URA 1160 Institut Pasteur, Lille, France. Search for more papers by this author J. F. Duvall J. F. Duvall INSERM U186/CNRS URA 1160 Institut Pasteur, Lille, France. Search for more papers by this author A. Gégonne A. Gégonne INSERM U186/CNRS URA 1160 Institut Pasteur, Lille, France. Search for more papers by this author M. Bailly M. Bailly INSERM U186/CNRS URA 1160 Institut Pasteur, Lille, France. Search for more papers by this author A. Hémar A. Hémar INSERM U186/CNRS URA 1160 Institut Pasteur, Lille, France. Search for more papers by this author J. Brady J. Brady INSERM U186/CNRS URA 1160 Institut Pasteur, Lille, France. Search for more papers by this author J. Ghysdael J. Ghysdael INSERM U186/CNRS URA 1160 Institut Pasteur, Lille, France. Search for more papers by this author Author Information R. Bosselut1, J. F. Duvall1, A. Gégonne1, M. Bailly1, A. Hémar1, J. Brady1 and J. Ghysdael1 1INSERM U186/CNRS URA 1160 Institut Pasteur, Lille, France. The EMBO Journal (1990)9:3137-3144https://doi.org/10.1002/j.1460-2075.1990.tb07511.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info The c-ets-1 proto-oncogene and the related c-ets-2 gene encode related nuclear chromatin-associated proteins which bind DNA in vitro. To investigate the possibility that Ets1 and Ets2 are transcriptional activators, we analyzed the ability of these proteins to trans-activate promoter/enhancer sequences in transient co-transfection experiments. A CAT construct driven by the long terminal repeat of the human T cell leukemia virus, HTLV-1 was found to be trans-activated by both Ets1 and Ets2 in NIH3T3 and HeLa cells. The increased levels of CAT activity were paralleled by increased levels of correctly initiated CAT mRNA. Mutant Ets1 proteins unable to accumulate in the nucleus were found to be inactive. An ets-responsive sequence between positions −117 and −160 of the LTR was identified by analyses of a series of 5′ deletion mutants of the HTLV-1 LTR and of dimerized versions of specific motifs of the LTR enhancer region. Using a gel shift binding assay, Ets1 was found to bind specifically to an oligonucleotide corresponding to region −117 to −160. This sequence, which also contributes to Tax1 responsiveness of the HTLV-1 LTR, is characterized by the presence of four repeats of a pentanucleotide sequence of the type CC(T/A)CC. Competition experiments show that integrity of repeats 1 and 4 is important for Ets1 binding. These results show that Ets1 and Ets2 are sequence-specific transcriptional activators. In view of the high level expression of Ets1 in lymphoid cells, Ets1 could be part of the transcription complex which mediates the response to Tax1 and the control of HTLV-1 replication. More generally, Ets1 and Ets2 could regulate transcription of cellular genes. Previous ArticleNext Article Volume 9Issue 101 October 1990In this issue RelatedDetailsLoading ...